Simultaneous quantification of Pacific ciguatoxins in fish blood using liquid chromatography-tandem mass spectrometry

Yim Ling Mak, Jia Jun Wu, Wing Hei Chan, Margaret B. Murphy, James C.W. Lam, Leo L. Chan, Paul K.S. Lam

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Ciguatera fish poisoning (CFP) is a food intoxication caused by exposure to ciguatoxins (CTXs) in coral reef fish. Rapid analytical methods have been developed recently to quantify Pacific-CTX-1 (P-CTX-1) in fish muscle, but it is destructive and can cause harm to valuable live coral reef fish. Also fish muscle extract was complex making CTX quantification challenging. Not only P-CTX-1, but also P-CTX-2 and P-CTX-3 could be present in fish, contributing to ciguatoxicity. Therefore, an analytical method for simultaneous quantification of P-CTX-1, P-CTX-2, and P-CTX-3 in whole blood of marketed coral reef fish using sonication, solid-phase extraction (SPE), and liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. The optimized method gave acceptable recoveries of P-CTXs (74-103 %) in fish blood. Matrix effects (6-26 %) in blood extracts were found to be significantly reduced compared with those in muscle extracts (suppressed by 34-75 % as reported in other studies), thereby minimizing potential for false negative results. The target P-CTXs were detectable in whole blood from four coral reef fish species collected in a CFP-endemic region. Similar trends in total P-CTX levels and patterns of P-CTX composition profiles in blood and muscle of these fish were observed, suggesting a relationship between blood and muscle levels of P-CTXs. This optimized method provides an essential tool for studies of P-CTX pharmacokinetics and pharmacodynamics in fish, which are needed for establishing the use of fish blood as a reliable sample for the assessment and control of CFP. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)3331-3340
Number of pages10
JournalAnalytical and Bioanalytical Chemistry
Volume405
Issue number10
DOIs
Publication statusPublished - Apr 2013
Externally publishedYes

Keywords

  • Ciguatera fish poisoning
  • Fish blood
  • LC-MS/MS
  • P-CTXs
  • Pacific ciguatoxins

Cite this

Mak, Yim Ling ; Wu, Jia Jun ; Chan, Wing Hei ; Murphy, Margaret B. ; Lam, James C.W. ; Chan, Leo L. ; Lam, Paul K.S. / Simultaneous quantification of Pacific ciguatoxins in fish blood using liquid chromatography-tandem mass spectrometry. In: Analytical and Bioanalytical Chemistry. 2013 ; Vol. 405, No. 10. pp. 3331-3340.
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title = "Simultaneous quantification of Pacific ciguatoxins in fish blood using liquid chromatography-tandem mass spectrometry",
abstract = "Ciguatera fish poisoning (CFP) is a food intoxication caused by exposure to ciguatoxins (CTXs) in coral reef fish. Rapid analytical methods have been developed recently to quantify Pacific-CTX-1 (P-CTX-1) in fish muscle, but it is destructive and can cause harm to valuable live coral reef fish. Also fish muscle extract was complex making CTX quantification challenging. Not only P-CTX-1, but also P-CTX-2 and P-CTX-3 could be present in fish, contributing to ciguatoxicity. Therefore, an analytical method for simultaneous quantification of P-CTX-1, P-CTX-2, and P-CTX-3 in whole blood of marketed coral reef fish using sonication, solid-phase extraction (SPE), and liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. The optimized method gave acceptable recoveries of P-CTXs (74-103 {\%}) in fish blood. Matrix effects (6-26 {\%}) in blood extracts were found to be significantly reduced compared with those in muscle extracts (suppressed by 34-75 {\%} as reported in other studies), thereby minimizing potential for false negative results. The target P-CTXs were detectable in whole blood from four coral reef fish species collected in a CFP-endemic region. Similar trends in total P-CTX levels and patterns of P-CTX composition profiles in blood and muscle of these fish were observed, suggesting a relationship between blood and muscle levels of P-CTXs. This optimized method provides an essential tool for studies of P-CTX pharmacokinetics and pharmacodynamics in fish, which are needed for establishing the use of fish blood as a reliable sample for the assessment and control of CFP. [Figure not available: see fulltext.]",
keywords = "Ciguatera fish poisoning, Fish blood, LC-MS/MS, P-CTXs, Pacific ciguatoxins",
author = "Mak, {Yim Ling} and Wu, {Jia Jun} and Chan, {Wing Hei} and Murphy, {Margaret B.} and Lam, {James C.W.} and Chan, {Leo L.} and Lam, {Paul K.S.}",
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Simultaneous quantification of Pacific ciguatoxins in fish blood using liquid chromatography-tandem mass spectrometry. / Mak, Yim Ling; Wu, Jia Jun; Chan, Wing Hei; Murphy, Margaret B.; Lam, James C.W.; Chan, Leo L.; Lam, Paul K.S.

In: Analytical and Bioanalytical Chemistry, Vol. 405, No. 10, 04.2013, p. 3331-3340.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Mak, Yim Ling

AU - Wu, Jia Jun

AU - Chan, Wing Hei

AU - Murphy, Margaret B.

AU - Lam, James C.W.

AU - Chan, Leo L.

AU - Lam, Paul K.S.

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AB - Ciguatera fish poisoning (CFP) is a food intoxication caused by exposure to ciguatoxins (CTXs) in coral reef fish. Rapid analytical methods have been developed recently to quantify Pacific-CTX-1 (P-CTX-1) in fish muscle, but it is destructive and can cause harm to valuable live coral reef fish. Also fish muscle extract was complex making CTX quantification challenging. Not only P-CTX-1, but also P-CTX-2 and P-CTX-3 could be present in fish, contributing to ciguatoxicity. Therefore, an analytical method for simultaneous quantification of P-CTX-1, P-CTX-2, and P-CTX-3 in whole blood of marketed coral reef fish using sonication, solid-phase extraction (SPE), and liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed. The optimized method gave acceptable recoveries of P-CTXs (74-103 %) in fish blood. Matrix effects (6-26 %) in blood extracts were found to be significantly reduced compared with those in muscle extracts (suppressed by 34-75 % as reported in other studies), thereby minimizing potential for false negative results. The target P-CTXs were detectable in whole blood from four coral reef fish species collected in a CFP-endemic region. Similar trends in total P-CTX levels and patterns of P-CTX composition profiles in blood and muscle of these fish were observed, suggesting a relationship between blood and muscle levels of P-CTXs. This optimized method provides an essential tool for studies of P-CTX pharmacokinetics and pharmacodynamics in fish, which are needed for establishing the use of fish blood as a reliable sample for the assessment and control of CFP. [Figure not available: see fulltext.]

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