Simultaneous estimation of pregnenolone, 17-hydroxypregnenolone and dehydroepiandrosterone by gas-liquid radiochromatography

E. Nice; K. Williams

doi:10.1016/0003-2697(74)90291-7

Simultaneous estimation of pregnenolone, 17-hydroxypregnenolone and dehydroepiandrosterone by gas-liquid radiochromatography

E. Nice, K. Williams

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5 Citations (Scopus)

Abstract

Mixtures of the 5-ene-3β-hydroxysteroids pregnenolone, 17-hydroxypregnenolone and DNA have been simultaneously separated and quantitated by gas-liquid radiochromatography. Over 98% of the injected radioactivity was accounted for using an improved trapping technique. The identity of the recovered steroids was confirmed by mass spectrometry. The analytical method has been applied successfully to the analysis of the in vitro metabolism of pregnenolone by the microsomes of the human adrenal gland.

Original language	English
Pages (from-to)	399-406
Number of pages	8
Journal	Analytical Biochemistry
Volume	59
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(74)90291-7
Publication status	Published - 1 Jan 1974
Externally published	Yes

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title = "Simultaneous estimation of pregnenolone, 17-hydroxypregnenolone and dehydroepiandrosterone by gas-liquid radiochromatography",

abstract = "Mixtures of the 5-ene-3β-hydroxysteroids pregnenolone, 17-hydroxypregnenolone and DNA have been simultaneously separated and quantitated by gas-liquid radiochromatography. Over 98% of the injected radioactivity was accounted for using an improved trapping technique. The identity of the recovered steroids was confirmed by mass spectrometry. The analytical method has been applied successfully to the analysis of the in vitro metabolism of pregnenolone by the microsomes of the human adrenal gland.",

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N2 - Mixtures of the 5-ene-3β-hydroxysteroids pregnenolone, 17-hydroxypregnenolone and DNA have been simultaneously separated and quantitated by gas-liquid radiochromatography. Over 98% of the injected radioactivity was accounted for using an improved trapping technique. The identity of the recovered steroids was confirmed by mass spectrometry. The analytical method has been applied successfully to the analysis of the in vitro metabolism of pregnenolone by the microsomes of the human adrenal gland.

AB - Mixtures of the 5-ene-3β-hydroxysteroids pregnenolone, 17-hydroxypregnenolone and DNA have been simultaneously separated and quantitated by gas-liquid radiochromatography. Over 98% of the injected radioactivity was accounted for using an improved trapping technique. The identity of the recovered steroids was confirmed by mass spectrometry. The analytical method has been applied successfully to the analysis of the in vitro metabolism of pregnenolone by the microsomes of the human adrenal gland.

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ER -

Simultaneous estimation of pregnenolone, 17-hydroxypregnenolone and dehydroepiandrosterone by gas-liquid radiochromatography

Abstract

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