Simultaneous estimation of pregnenolone, 17-hydroxypregnenolone and dehydroepiandrosterone by gas-liquid radiochromatography

E. Nice, K. Williams

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Mixtures of the 5-ene-3β-hydroxysteroids pregnenolone, 17-hydroxypregnenolone and DNA have been simultaneously separated and quantitated by gas-liquid radiochromatography. Over 98% of the injected radioactivity was accounted for using an improved trapping technique. The identity of the recovered steroids was confirmed by mass spectrometry. The analytical method has been applied successfully to the analysis of the in vitro metabolism of pregnenolone by the microsomes of the human adrenal gland.

Original languageEnglish
Pages (from-to)399-406
Number of pages8
JournalAnalytical Biochemistry
Issue number2
Publication statusPublished - 1 Jan 1974
Externally publishedYes

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