Simultaneous determination of betamethasone, betamethasone acetate and hydrocortisone in biological fluids using high-performance liquid chromatography

Marisa C. Petersen, Roger L. Nation, John J. Ashley

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A sensitive, specific, reproducible and rapid high-performance liquid chromatographic method for the simultaneous quantitation of betamethasone, betamethasone 21-acetate and hydrocortisone in biological fluids is described. Hydrocortisone acetate is used as an internal standard and the samples are extracted with dichloromethane before chromatographing on a reversed-phase system. Detection at two ultraviolet wavelengths (254 nm and 240 nm) was used to assess the specificity of the system, and the sensitivity was found to be greater than 10 ng for all steroids. The speed with which this assay can be performed makes it particularly useful for pharmacokinetic studies, and plasma concentration—time profiles resulting from the administration of betamethasone phosphate and betamethasone acetate are presented.

Original languageEnglish
Pages (from-to)131-139
Number of pages9
JournalJournal of Chromatography. Biomedical Applications
Issue number2
Publication statusPublished - 1 Jan 1980

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