Simultaneous determination of 6-monoacetylmorphine, morphine and codeine in urine using high-performance liquid chromatography with combined ultraviolet and electrochemical detection

Jim Gerostamoulos, Kerryn Crump, Iain M. McIntyre, Olaf H. Drummer

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A method is described for the simultaneous determination of 6-monoacetylmorphine (6-MAM), morphine and codeine in post-mortem urine specimens using reversed-phase high-performance liquid chromatography with dual ultraviolet spectrophotometric and electrochemical detection. The limits of detection for a 1-ml urine sample were 0.04 mg/l for 6-MAM and 0.05 mg/l for both morphine and codeine. The presence of 6-MAM in urine indicates prior use of heroin and enables differentiation between morphine- and heroin-related deaths.

Original languageEnglish
Pages (from-to)152-156
Number of pages5
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number1
Publication statusPublished - 23 Jul 1993

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