Simultaneous ATR-FTIR Based Determination of Malaria Parasitemia, Glucose and Urea in Whole Blood Dried onto a Glass Slide

Supti Roy, David Perez-Guaita, Dean W Andrew, Jack S. Richards, Don McNaughton, Philip Heraud, Bayden R. Wood

Research output: Contribution to journalArticleResearchpeer-review

Abstract

New diagnostic tools that can detect malaria parasites in conjunction with other diagnostic parameters are urgently required. In this study, Attenuated Total Reflection Fourier transform infrared (ATR-FTIR) spectroscopy in combination with Partial Least Square Discriminant Analysis (PLS-DA) and Partial Least Square Regression (PLS-R) have been applied as a point-of-care test for identifying malaria parasites, blood glucose, and urea levels in whole blood samples from thick blood films on glass slides. The specificity for the PLS-DA was found to be 98% for parasitemia levels >0.5%, but a rather low sensitivity of 70% was achieved because of the small number of negative samples in the model. In PLS-R the Root Mean Square Error of Cross Validation (RMSECV) for parasite concentration (0-5%) was 0.58%. Similarly, for glucose (0-400 mg/dL) and urea (0-250 mg/dL) spiked samples, relative RMSECVs were 16% and 17%, respectively. The method reported here is the first example of multianalyte/disease diagnosis using ATR-FTIR spectroscopy, which in this case, enabled the simultaneous quantification of glucose and urea analytes along with malaria parasitemia quantification using one spectrum obtained from a single drop of blood on a glass microscope slide.

Original languageEnglish
Pages (from-to)5238-5245
Number of pages8
JournalAnalytical Chemistry
Volume89
Issue number10
DOIs
Publication statusPublished - 16 May 2017

Cite this

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title = "Simultaneous ATR-FTIR Based Determination of Malaria Parasitemia, Glucose and Urea in Whole Blood Dried onto a Glass Slide",
abstract = "New diagnostic tools that can detect malaria parasites in conjunction with other diagnostic parameters are urgently required. In this study, Attenuated Total Reflection Fourier transform infrared (ATR-FTIR) spectroscopy in combination with Partial Least Square Discriminant Analysis (PLS-DA) and Partial Least Square Regression (PLS-R) have been applied as a point-of-care test for identifying malaria parasites, blood glucose, and urea levels in whole blood samples from thick blood films on glass slides. The specificity for the PLS-DA was found to be 98{\%} for parasitemia levels >0.5{\%}, but a rather low sensitivity of 70{\%} was achieved because of the small number of negative samples in the model. In PLS-R the Root Mean Square Error of Cross Validation (RMSECV) for parasite concentration (0-5{\%}) was 0.58{\%}. Similarly, for glucose (0-400 mg/dL) and urea (0-250 mg/dL) spiked samples, relative RMSECVs were 16{\%} and 17{\%}, respectively. The method reported here is the first example of multianalyte/disease diagnosis using ATR-FTIR spectroscopy, which in this case, enabled the simultaneous quantification of glucose and urea analytes along with malaria parasitemia quantification using one spectrum obtained from a single drop of blood on a glass microscope slide.",
author = "Supti Roy and David Perez-Guaita and Andrew, {Dean W} and Richards, {Jack S.} and Don McNaughton and Philip Heraud and Wood, {Bayden R.}",
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language = "English",
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Simultaneous ATR-FTIR Based Determination of Malaria Parasitemia, Glucose and Urea in Whole Blood Dried onto a Glass Slide. / Roy, Supti; Perez-Guaita, David; Andrew, Dean W; Richards, Jack S.; McNaughton, Don; Heraud, Philip; Wood, Bayden R.

In: Analytical Chemistry, Vol. 89, No. 10, 16.05.2017, p. 5238-5245.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Simultaneous ATR-FTIR Based Determination of Malaria Parasitemia, Glucose and Urea in Whole Blood Dried onto a Glass Slide

AU - Roy, Supti

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AU - Andrew, Dean W

AU - Richards, Jack S.

AU - McNaughton, Don

AU - Heraud, Philip

AU - Wood, Bayden R.

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