Abstract
A rapid high‐pressure liquid chromatographic assay is described for the quantitative determination of griseofulvin in plasma. An aliquot (25‐100 μl) of plasma was deproteinized by a simple procedure involving the addition of 2.5 volumes of acetonitrile, vortex mixing for a few seconds, and centrifugation for 1 min. The clear supernate, 50 μl, was injected into the high‐pressure liquid chromatograph. A reversed‐phase column was used with a mobile phase of distilled water‐acetonitrile (1:1) at a flow rate of 2 ml/min and was operated at ambient temperature. A fluorescent detector with an excitation wavelength of 260 nm was employed to monitor the column effluent. Griseofulvin had a retention time of 3.8 min. This procedure yields reproducible results with high sensitivity; plasma concentrations as low as 50 ng/ml can be measured. Several commonly used drugs do not interfere. Analysis of plasma samples collected from a rabbit injected with griseofulvin indicated that the procedure is suitable for pharmacokinetic studies and clinical monitoring of plasma concentrations in patients. Assay turnaround time is less than 6 min. For clinical monitoring of plasma griseofulvin concentrations, a sample volume as small as 10 μl can be used.
| Original language | English |
|---|---|
| Pages (from-to) | 805-808 |
| Number of pages | 4 |
| Journal | Journal of Pharmaceutical Sciences |
| Volume | 67 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1 Jan 1978 |
Keywords
- Antifungal agents—griseofulvin, high‐pressure liquid chromatographic analysis in plasma
- Griseofulvin—high‐pressure liquid chromatographic analysis in plasma
- High‐pressure liquid chromatography—analysis, griseofulvin in plasma
Cite this
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Simple, rapid, and micro high‐pressure liquid chromatographic determination of plasma griseofulvin levels. / Nation, Roger L.; Peng, Geoffrey W.; Smith, Vicky; Chiou, Win L.
In: Journal of Pharmaceutical Sciences, Vol. 67, No. 6, 01.01.1978, p. 805-808.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Simple, rapid, and micro high‐pressure liquid chromatographic determination of plasma griseofulvin levels
AU - Nation, Roger L.
AU - Peng, Geoffrey W.
AU - Smith, Vicky
AU - Chiou, Win L.
PY - 1978/1/1
Y1 - 1978/1/1
N2 - A rapid high‐pressure liquid chromatographic assay is described for the quantitative determination of griseofulvin in plasma. An aliquot (25‐100 μl) of plasma was deproteinized by a simple procedure involving the addition of 2.5 volumes of acetonitrile, vortex mixing for a few seconds, and centrifugation for 1 min. The clear supernate, 50 μl, was injected into the high‐pressure liquid chromatograph. A reversed‐phase column was used with a mobile phase of distilled water‐acetonitrile (1:1) at a flow rate of 2 ml/min and was operated at ambient temperature. A fluorescent detector with an excitation wavelength of 260 nm was employed to monitor the column effluent. Griseofulvin had a retention time of 3.8 min. This procedure yields reproducible results with high sensitivity; plasma concentrations as low as 50 ng/ml can be measured. Several commonly used drugs do not interfere. Analysis of plasma samples collected from a rabbit injected with griseofulvin indicated that the procedure is suitable for pharmacokinetic studies and clinical monitoring of plasma concentrations in patients. Assay turnaround time is less than 6 min. For clinical monitoring of plasma griseofulvin concentrations, a sample volume as small as 10 μl can be used.
AB - A rapid high‐pressure liquid chromatographic assay is described for the quantitative determination of griseofulvin in plasma. An aliquot (25‐100 μl) of plasma was deproteinized by a simple procedure involving the addition of 2.5 volumes of acetonitrile, vortex mixing for a few seconds, and centrifugation for 1 min. The clear supernate, 50 μl, was injected into the high‐pressure liquid chromatograph. A reversed‐phase column was used with a mobile phase of distilled water‐acetonitrile (1:1) at a flow rate of 2 ml/min and was operated at ambient temperature. A fluorescent detector with an excitation wavelength of 260 nm was employed to monitor the column effluent. Griseofulvin had a retention time of 3.8 min. This procedure yields reproducible results with high sensitivity; plasma concentrations as low as 50 ng/ml can be measured. Several commonly used drugs do not interfere. Analysis of plasma samples collected from a rabbit injected with griseofulvin indicated that the procedure is suitable for pharmacokinetic studies and clinical monitoring of plasma concentrations in patients. Assay turnaround time is less than 6 min. For clinical monitoring of plasma griseofulvin concentrations, a sample volume as small as 10 μl can be used.
KW - Antifungal agents—griseofulvin, high‐pressure liquid chromatographic analysis in plasma
KW - Griseofulvin—high‐pressure liquid chromatographic analysis in plasma
KW - High‐pressure liquid chromatography—analysis, griseofulvin in plasma
UR - http://www.scopus.com/inward/record.url?scp=0017815294&partnerID=8YFLogxK
U2 - 10.1002/jps.2600670620
DO - 10.1002/jps.2600670620
M3 - Article
VL - 67
SP - 805
EP - 808
JO - Journal of Pharmaceutical Sciences
JF - Journal of Pharmaceutical Sciences
SN - 0022-3549
IS - 6
ER -