Projects per year
Abstract
The study of the mitochondrial respiratory chain (MRC) function in relation with its structural organization is of great interest due to the central role of this system in eukaryotic cell metabolism. The complexome profiling technique has provided invaluable information for our understanding of the composition and assembly of the individual MRC complexes, and also of their association into larger supercomplexes (SCs) and respirasomes. The formation of the SCs has been highly debated, and their assembly and regulation mechanisms are still unclear. Previous studies demonstrated a prominent role for COX7A2L (SCAFI) as a structural protein bridging the association of individual MRC complexes III and IV in the minor SC III2 + IV, although its relevance for respirasome formation and function remains controversial. In this work, we have used SILAC-based complexome profiling to dissect the structural organization of the human MRC in HEK293T cells depleted of SCAFI (SCAFIKO) by CRISPR-Cas9 genome editing. SCAFI ablation led to a preferential loss of SC III2 + IV and of a minor subset of respirasomes without affecting OXPHOS function. Our data suggest that the loss of SCAFI-dependent respirasomes in SCAFIKO cells is mainly due to alterations on early stages of CI assembly, without impacting the biogenesis of complexes III and IV. Contrary to the idea of SCAFI being the main player in respirasome formation, SILAC-complexome profiling showed that, in wild-type cells, the majority of respirasomes (ca. 70%) contained COX7A2 and that these species were present at roughly the same levels when SCAFI was knocked-out. We thus demonstrate the co-existence of structurally distinct respirasomes defined by the preferential binding of complex IV via COX7A2, rather than SCAFI, in human cultured cells.
Original language | English |
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Article number | 148414 |
Number of pages | 11 |
Journal | BBA Bioenergetics |
Volume | 1862 |
Issue number | 7 |
DOIs | |
Publication status | Published - 1 Jul 2021 |
Keywords
- COX7A2
- COX7A2L/SCAFI
- Mitochondria
- oxidative phosphorylation
- respiratory chain
- respiratory supercomplexes
Projects
- 4 Finished
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Defining molecular pathways for COX2 maturation in mitochondrial Complex IV
Ryan, M., Maher, M. J. & Kwan, A.
National Health and Medical Research Council (NHMRC) (Australia)
1/01/19 → 31/12/22
Project: Research
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Systems approaches to understanding the assembly of mitochondrial machines
Stroud, D. & Ryan, M.
1/01/18 → 31/12/20
Project: Research