Aims: To investigate the relative role of the red dry and rough (rdar) and brown dry and rough (bdar) morphotypes on hydrophobicity and ability to attach to abiotic surfaces of poultry-associated Salmonella strains with a focus on S. Sofia. Methods and Results: Cellulose synthase gene null mutants were constructed in five Salmonella strains converting them from rdar to bdar morphotypes. One S. Sofia null mutant displayed reduced hydrophobicity and attachment to Teflon (R) relative to its parent strain. The S. Virchow and S. Infantis null mutants attached less well to glass relative to their parent strains. Conclusions: The rdar or bdar morphotype may influence S. Sofia persistence but did not explain why bdar strains predominate in this serotype. Significance and Impact of the Study: This work provides some insight into why some Salmonella strains survive in poultry environments and may ultimately contribute to their control.