Short report: Production of recombinant kinesin-related protein of Leishmania donovani and its application in the serodiagnosis of visceral leishmaniasis

Hidekazu Takagi, Mohammad Zahidul Islam, Makoto Itoh, Anwar Ul Islam, A. R.M.Saifuddin Ekram, Sultana Monira Hussain, Yoshihisa Hashiguchi, Eisaku Kimura

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23 Citations (Scopus)

Abstract

To detect IgG antibody in the serodiagnosis of visceral leishmaniasis (VL), a recombinant antigen rK39, which is part of a Leishmania chagasi kinesin-related protein, has been used successfully and showed high sensitivity and specificity. We report production of a recombinant protein rKRP42, which is part of an L. donovani kinesin-related protein and a homolog of rK39, and its application in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of VL. When rKRP42 and rK39 were compared, amino acid sequence analysis showed 89.3% identity and 98.7% homology, with rKRP42 having 39 more amino acids than rK39. The ELISA using rKRP42 showed a sensitivity of 94.6% (70 positive samples among 74 from VL patients) and a specificity of 99.3% (148 negative samples among 149 samples from Japanese controls), whereas the sensitivity of the commercial rK39 dipstick test was 93.2% (69 positive samples among 74 from patients with VL). The rKRP42 is a promising new antigen in developing immunodiagnostic methods for VL.

Original languageEnglish
Pages (from-to)902-905
Number of pages4
JournalThe American Journal of Tropical Medicine and Hygiene
Volume76
Issue number5
Publication statusPublished - 1 May 2007
Externally publishedYes

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