Abstract
Thermally-responsive, short elastin-like peptides (ELPs) of sequence VPGVG (V, P and G represent valine, proline and glycine respectively), bearing different N-terminal functional groups (amino-, N-acetyl and thiol) and a non-ionisable C-terminal group, were prepared by solid phase synthesis. The conformation and aggregation properties of the ELPs were studied in different pH aqueous buffer solutions using UV-vis spectroscopy and circular dichroism (CD). The thiol-capped ELPs were used to prepare functionalized gold nanoparticles (GNPs), which were found to undergo thermally-triggered reversible aggregation at 40 °C. The peptide conformation and nanoparticle aggregation behaviour of the ELP-GNPs in aqueous solution were investigated by transmission electron microscopy (TEM), circular dichroism (CD) and UV-vis spectroscopy. It was found that the ELP-GNP conjugates were capable of reversible, thermally triggered aggregation at near-physiological temperatures (transition temperature of 40 °C at pH = 7.4), opening up applications in photothermal cancer therapy and diagnosis.
| Original language | English |
|---|---|
| Pages (from-to) | 6667-6674 |
| Number of pages | 8 |
| Journal | Journal of Materials Chemistry B |
| Volume | 6 |
| Issue number | 41 |
| DOIs | |
| Publication status | Published - 7 Nov 2018 |
Cite this
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Short elastin-like peptide-functionalized gold nanoparticles that are temperature responsive under near-physiological conditions. / Zong, Jingyi; Cobb, Steven L.; Cameron, Neil R.
In: Journal of Materials Chemistry B, Vol. 6, No. 41, 07.11.2018, p. 6667-6674.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Short elastin-like peptide-functionalized gold nanoparticles that are temperature responsive under near-physiological conditions
AU - Zong, Jingyi
AU - Cobb, Steven L.
AU - Cameron, Neil R.
PY - 2018/11/7
Y1 - 2018/11/7
N2 - Thermally-responsive, short elastin-like peptides (ELPs) of sequence VPGVG (V, P and G represent valine, proline and glycine respectively), bearing different N-terminal functional groups (amino-, N-acetyl and thiol) and a non-ionisable C-terminal group, were prepared by solid phase synthesis. The conformation and aggregation properties of the ELPs were studied in different pH aqueous buffer solutions using UV-vis spectroscopy and circular dichroism (CD). The thiol-capped ELPs were used to prepare functionalized gold nanoparticles (GNPs), which were found to undergo thermally-triggered reversible aggregation at 40 °C. The peptide conformation and nanoparticle aggregation behaviour of the ELP-GNPs in aqueous solution were investigated by transmission electron microscopy (TEM), circular dichroism (CD) and UV-vis spectroscopy. It was found that the ELP-GNP conjugates were capable of reversible, thermally triggered aggregation at near-physiological temperatures (transition temperature of 40 °C at pH = 7.4), opening up applications in photothermal cancer therapy and diagnosis.
AB - Thermally-responsive, short elastin-like peptides (ELPs) of sequence VPGVG (V, P and G represent valine, proline and glycine respectively), bearing different N-terminal functional groups (amino-, N-acetyl and thiol) and a non-ionisable C-terminal group, were prepared by solid phase synthesis. The conformation and aggregation properties of the ELPs were studied in different pH aqueous buffer solutions using UV-vis spectroscopy and circular dichroism (CD). The thiol-capped ELPs were used to prepare functionalized gold nanoparticles (GNPs), which were found to undergo thermally-triggered reversible aggregation at 40 °C. The peptide conformation and nanoparticle aggregation behaviour of the ELP-GNPs in aqueous solution were investigated by transmission electron microscopy (TEM), circular dichroism (CD) and UV-vis spectroscopy. It was found that the ELP-GNP conjugates were capable of reversible, thermally triggered aggregation at near-physiological temperatures (transition temperature of 40 °C at pH = 7.4), opening up applications in photothermal cancer therapy and diagnosis.
UR - http://www.scopus.com/inward/record.url?scp=85055726454&partnerID=8YFLogxK
U2 - 10.1039/c8tb01827h
DO - 10.1039/c8tb01827h
M3 - Article
VL - 6
SP - 6667
EP - 6674
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
SN - 2050-750X
IS - 41
ER -