LIM1215 colon cancer cells were used as a model of human colonic epithelium to examine the effects of butyrate on protein kinase C (PKC) activity and isoform expression. On Western blot analysis, LIM1215 cells express the PKC isoforms α, β, ε, ζ, and λ, but not γ, θ, or μ. Treatment with 2 mM butyrate for 48 h reduced cellular PKC activity up to 50% and specifically reduced the expression of PKCα and PKCε. Similar results were obtained using Caco-2 colon cancer cells. These effects were neither a consequence of the induction of differentiation itself nor the result of direct or indirect activation of PKC. Although dependent on gene transcription and protein synthesis, the effect was not due to a reduction in the synthesis of PKC protein. Butyrate's effect was independent of its β- oxidation but was mimicked, at least in part, by trichostatin A, an inhibitor of histone deacetylase.
|Number of pages||10|
|Journal||Journal of Cellular Physiology|
|Publication status||Published - 13 Jan 2000|