Three monoclonal antibodies, SBU.I41-17, 41-19 and 41-28, have been produced which recognize sheep class I major histocompatibility complex antigens (OLA). All three antibodies are able to precipitate a heavy chain of 44,000 MW and a smaller β2-microglobulin of 12,000 MW from 125I-surface labelled lymphocytes. The antibodies have been used to localize sheep OLA Class I antigens in lymphoid and non-lymphoid tissues using indirect immunoperoxidase histological staining and cytofluorograph analyses. Evidence suggests that the three antibodies are directed against monomorphic determinants but that they recognize different epitopes.
|Number of pages||7|
|Publication status||Published - 1 Dec 1985|