Sheep lymphocyte antigens (OLA). I. Major histocompatibility complex class I molecules

K. J. Gogolin-Ewens, C. R. Mackay, W. R. Mercer, M. R. Brandon

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Abstract

Three monoclonal antibodies, SBU.I41-17, 41-19 and 41-28, have been produced which recognize sheep class I major histocompatibility complex antigens (OLA). All three antibodies are able to precipitate a heavy chain of 44,000 MW and a smaller β2-microglobulin of 12,000 MW from 125I-surface labelled lymphocytes. The antibodies have been used to localize sheep OLA Class I antigens in lymphoid and non-lymphoid tissues using indirect immunoperoxidase histological staining and cytofluorograph analyses. Evidence suggests that the three antibodies are directed against monomorphic determinants but that they recognize different epitopes.

Original languageEnglish
Pages (from-to)717-723
Number of pages7
JournalImmunology
Volume56
Issue number4
Publication statusPublished - 1 Dec 1985
Externally publishedYes

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