Sheep hypothalamus contains a non-angiotensin ligand for type 1 and type 2 angiotensin II receptors

Julie Blasioli; Athena Kladis; Iain J. Clarke; Duncan J. Campbell

doi:10.1111/j.1440-1681.1993.tb01741.x

Sheep hypothalamus contains a non-angiotensin ligand for type 1 and type 2 angiotensin II receptors

Julie Blasioli, Athena Kladis, Iain J. Clarke, Duncan J. Campbell

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

1. The aim of this study was to determine whether the brain contains an alternative ligand for angiotensin II (AII) receptors. 2. A radioreceptor assay based upon bovine cerebellar membranes (Type 2 AII receptors) was used to monitor the partial purification of an AII‐like material from sheep hypothalami. 3. This material displaces ¹²⁵I‐[Sar¹, Ala⁸I‐AII from both type 1 (rat adrenal capsuIar membranes) and Type 2 AII receptors in a manner parallel to that of AIL It has a size of ∼30000 Da, is strongly cationic, is stable to boiling but is destroyed by trypsin. It is not recognized by AII antisera. 4. These data provide direct evidence for a non‐angiotensin endogenous ligand for brain AII receptors. This novel ligand may play a role in the regulation of blood pressure and other actions mediated by brain AII receptors.

Original language	English
Pages (from-to)	555-562
Number of pages	8
Journal	Clinical and Experimental Pharmacology and Physiology
Volume	20
Issue number	9
DOIs	https://doi.org/10.1111/j.1440-1681.1993.tb01741.x
Publication status	Published - 1 Jan 1993
Externally published	Yes

Keywords

angiotensin II receptors
endogenous ligand
hypothalamus
sheep brain.

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10.1111/j.1440-1681.1993.tb01741.x

Cite this

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title = "Sheep hypothalamus contains a non-angiotensin ligand for type 1 and type 2 angiotensin II receptors",

abstract = "1. The aim of this study was to determine whether the brain contains an alternative ligand for angiotensin II (AII) receptors. 2. A radioreceptor assay based upon bovine cerebellar membranes (Type 2 AII receptors) was used to monitor the partial purification of an AII‐like material from sheep hypothalami. 3. This material displaces 125I‐[Sar1, Ala8I‐AII from both type 1 (rat adrenal capsuIar membranes) and Type 2 AII receptors in a manner parallel to that of AIL It has a size of ∼30000 Da, is strongly cationic, is stable to boiling but is destroyed by trypsin. It is not recognized by AII antisera. 4. These data provide direct evidence for a non‐angiotensin endogenous ligand for brain AII receptors. This novel ligand may play a role in the regulation of blood pressure and other actions mediated by brain AII receptors.",

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N2 - 1. The aim of this study was to determine whether the brain contains an alternative ligand for angiotensin II (AII) receptors. 2. A radioreceptor assay based upon bovine cerebellar membranes (Type 2 AII receptors) was used to monitor the partial purification of an AII‐like material from sheep hypothalami. 3. This material displaces 125I‐[Sar1, Ala8I‐AII from both type 1 (rat adrenal capsuIar membranes) and Type 2 AII receptors in a manner parallel to that of AIL It has a size of ∼30000 Da, is strongly cationic, is stable to boiling but is destroyed by trypsin. It is not recognized by AII antisera. 4. These data provide direct evidence for a non‐angiotensin endogenous ligand for brain AII receptors. This novel ligand may play a role in the regulation of blood pressure and other actions mediated by brain AII receptors.

AB - 1. The aim of this study was to determine whether the brain contains an alternative ligand for angiotensin II (AII) receptors. 2. A radioreceptor assay based upon bovine cerebellar membranes (Type 2 AII receptors) was used to monitor the partial purification of an AII‐like material from sheep hypothalami. 3. This material displaces 125I‐[Sar1, Ala8I‐AII from both type 1 (rat adrenal capsuIar membranes) and Type 2 AII receptors in a manner parallel to that of AIL It has a size of ∼30000 Da, is strongly cationic, is stable to boiling but is destroyed by trypsin. It is not recognized by AII antisera. 4. These data provide direct evidence for a non‐angiotensin endogenous ligand for brain AII receptors. This novel ligand may play a role in the regulation of blood pressure and other actions mediated by brain AII receptors.

KW - angiotensin II receptors

KW - endogenous ligand

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Sheep hypothalamus contains a non-angiotensin ligand for type 1 and type 2 angiotensin II receptors

Abstract

Keywords

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