Sheep hypothalamus contains a non-angiotensin ligand for type 1 and type 2 angiotensin II receptors

Julie Blasioli, Athena Kladis, Iain J. Clarke, Duncan J. Campbell

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)


1. The aim of this study was to determine whether the brain contains an alternative ligand for angiotensin II (AII) receptors. 2. A radioreceptor assay based upon bovine cerebellar membranes (Type 2 AII receptors) was used to monitor the partial purification of an AII‐like material from sheep hypothalami. 3. This material displaces 125I‐[Sar1, Ala8I‐AII from both type 1 (rat adrenal capsuIar membranes) and Type 2 AII receptors in a manner parallel to that of AIL It has a size of ∼30000 Da, is strongly cationic, is stable to boiling but is destroyed by trypsin. It is not recognized by AII antisera. 4. These data provide direct evidence for a non‐angiotensin endogenous ligand for brain AII receptors. This novel ligand may play a role in the regulation of blood pressure and other actions mediated by brain AII receptors.

Original languageEnglish
Pages (from-to)555-562
Number of pages8
JournalClinical and Experimental Pharmacology and Physiology
Issue number9
Publication statusPublished - 1 Jan 1993
Externally publishedYes


  • angiotensin II receptors
  • endogenous ligand
  • hypothalamus
  • sheep brain.

Cite this