1. The aim of this study was to determine whether the brain contains an alternative ligand for angiotensin II (AII) receptors. 2. A radioreceptor assay based upon bovine cerebellar membranes (Type 2 AII receptors) was used to monitor the partial purification of an AII‐like material from sheep hypothalami. 3. This material displaces 125I‐[Sar1, Ala8I‐AII from both type 1 (rat adrenal capsuIar membranes) and Type 2 AII receptors in a manner parallel to that of AIL It has a size of ∼30000 Da, is strongly cationic, is stable to boiling but is destroyed by trypsin. It is not recognized by AII antisera. 4. These data provide direct evidence for a non‐angiotensin endogenous ligand for brain AII receptors. This novel ligand may play a role in the regulation of blood pressure and other actions mediated by brain AII receptors.
|Number of pages||8|
|Journal||Clinical and Experimental Pharmacology and Physiology|
|Publication status||Published - 1 Jan 1993|
- angiotensin II receptors
- endogenous ligand
- sheep brain.