Sex-specific young adult reference ranges for sex hormone concentrations measured on the Siemens ADVIA Centaur

Background: The present study aims to determine reference ranges for sex hormone concentrations measured on the Siemens ADVIA Centaur ® XP immunology analyzer system. Methods: The study sample consisted of 1638 individuals (814 men and 824 women) aged 18 - 60 years with measured serum concentrations of total testosterone (TT), sex hormonebinding globulin (SHBG), and dehydroepiandrosterone sulfate (DHEAS). Values for free testosterone (free T) and free androgen index (FAI) were calculated. Sex-and age-specific (18 to < 25, 25 to < 35, 35 to < 45, and = 45 years) reference ranges for these sex hormones were determined using quantile regression models for each sex hormone separately. Results: Sex hormone reference ranges were determined across each single year of age separately for men (TT: 5.60 - 29.58 nmol/L, SHBG: 17.65 - 73.64 nmol/L, DHEAS: 0.96 - 4.43 mg/L, free T: 0.10 - 0.51 nmol/L, and FAI: 15.04 - 70.37 nmol/L) and women (TT: 0.77 - 2.85 nmol/L, SHBG: 27.06 - 262.76 nmol/L, DHEAS: 0.50 - 3.15 mg/L, free T: 0.005 - 0.05 nmol/L, and FAI: 0.51 - 8.30 nmol/L), respectively.