Sex-and age-related differences in the chronic pressure-natriuresis relationship: role of the angiotensin type 2 receptor

Katrina M Mirabito, Lucinda M Hilliard, Michelle M Kett, Russell D Brown, Sean C Booth, Robert E Widdop, Karen M Moritz, Roger G Evans, Katherine M Denton

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30 Citations (Scopus)

Abstract

Sex hormones regulate the renin angiotensin system. For example, estrogen enhances expression of the angiotensin type 2 receptor. We hypothesized that activation of the angiotensin type 2 receptor shifts the chronic pressure-natriuresis relationship leftward in females compared to males, and that this effect is lost with age. Mean arterial pressure was measured by radiotelemetry in adult (4 month old) and aged (14 month old) wild type and angiotensin type 2 receptor knockout male and female mice. Chronic pressure-natriuresis curves were constructed whilst mice were maintained on a normal salt (0.26 ) diet and following 6 days of high salt (5.0 ) diet. Mean arterial pressure was lower in adult wild type females than males (88+/-1 and 97+/-1 mmHg respectively), a difference that was maintained with age, but was absent in adult knockout mice. In wild-type females, the chronic pressure-natriuresis relationship was shifted leftward compared to knockout females, an effect that was lost with age. In males, the chronic pressure-natriuresis relationship was not influenced by angiotensin type 2 receptor deficiency. Compared to age-matched females, the chronic pressure-natriuresis relationships of male mice were shifted rightward. Renal expression of the angiotensin type 2 receptor was 4-fold greater in adult wild type females than males. With age, the angiotensin type 2 receptor to angiotensin type 1 receptor balance was reduced in females. Conversely, in males, angiotensin receptor expression did not vary significantly with age. In conclusion, the angiotensin type 2 receptor modulates the chronic pressure-natriuresis relationship in an age and sex-dependent manner.
Original languageEnglish
Pages (from-to)901 - 907
Number of pages7
JournalAmerican Journal of Physiology - Renal Physiology
Volume307
Issue number8
DOIs
Publication statusPublished - 2014

Cite this

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title = "Sex-and age-related differences in the chronic pressure-natriuresis relationship: role of the angiotensin type 2 receptor",
abstract = "Sex hormones regulate the renin angiotensin system. For example, estrogen enhances expression of the angiotensin type 2 receptor. We hypothesized that activation of the angiotensin type 2 receptor shifts the chronic pressure-natriuresis relationship leftward in females compared to males, and that this effect is lost with age. Mean arterial pressure was measured by radiotelemetry in adult (4 month old) and aged (14 month old) wild type and angiotensin type 2 receptor knockout male and female mice. Chronic pressure-natriuresis curves were constructed whilst mice were maintained on a normal salt (0.26 ) diet and following 6 days of high salt (5.0 ) diet. Mean arterial pressure was lower in adult wild type females than males (88+/-1 and 97+/-1 mmHg respectively), a difference that was maintained with age, but was absent in adult knockout mice. In wild-type females, the chronic pressure-natriuresis relationship was shifted leftward compared to knockout females, an effect that was lost with age. In males, the chronic pressure-natriuresis relationship was not influenced by angiotensin type 2 receptor deficiency. Compared to age-matched females, the chronic pressure-natriuresis relationships of male mice were shifted rightward. Renal expression of the angiotensin type 2 receptor was 4-fold greater in adult wild type females than males. With age, the angiotensin type 2 receptor to angiotensin type 1 receptor balance was reduced in females. Conversely, in males, angiotensin receptor expression did not vary significantly with age. In conclusion, the angiotensin type 2 receptor modulates the chronic pressure-natriuresis relationship in an age and sex-dependent manner.",
author = "Mirabito, {Katrina M} and Hilliard, {Lucinda M} and Kett, {Michelle M} and Brown, {Russell D} and Booth, {Sean C} and Widdop, {Robert E} and Moritz, {Karen M} and Evans, {Roger G} and Denton, {Katherine M}",
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language = "English",
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Sex-and age-related differences in the chronic pressure-natriuresis relationship: role of the angiotensin type 2 receptor. / Mirabito, Katrina M; Hilliard, Lucinda M; Kett, Michelle M; Brown, Russell D; Booth, Sean C; Widdop, Robert E; Moritz, Karen M; Evans, Roger G; Denton, Katherine M.

In: American Journal of Physiology - Renal Physiology, Vol. 307, No. 8, 2014, p. 901 - 907.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Sex-and age-related differences in the chronic pressure-natriuresis relationship: role of the angiotensin type 2 receptor

AU - Mirabito, Katrina M

AU - Hilliard, Lucinda M

AU - Kett, Michelle M

AU - Brown, Russell D

AU - Booth, Sean C

AU - Widdop, Robert E

AU - Moritz, Karen M

AU - Evans, Roger G

AU - Denton, Katherine M

PY - 2014

Y1 - 2014

N2 - Sex hormones regulate the renin angiotensin system. For example, estrogen enhances expression of the angiotensin type 2 receptor. We hypothesized that activation of the angiotensin type 2 receptor shifts the chronic pressure-natriuresis relationship leftward in females compared to males, and that this effect is lost with age. Mean arterial pressure was measured by radiotelemetry in adult (4 month old) and aged (14 month old) wild type and angiotensin type 2 receptor knockout male and female mice. Chronic pressure-natriuresis curves were constructed whilst mice were maintained on a normal salt (0.26 ) diet and following 6 days of high salt (5.0 ) diet. Mean arterial pressure was lower in adult wild type females than males (88+/-1 and 97+/-1 mmHg respectively), a difference that was maintained with age, but was absent in adult knockout mice. In wild-type females, the chronic pressure-natriuresis relationship was shifted leftward compared to knockout females, an effect that was lost with age. In males, the chronic pressure-natriuresis relationship was not influenced by angiotensin type 2 receptor deficiency. Compared to age-matched females, the chronic pressure-natriuresis relationships of male mice were shifted rightward. Renal expression of the angiotensin type 2 receptor was 4-fold greater in adult wild type females than males. With age, the angiotensin type 2 receptor to angiotensin type 1 receptor balance was reduced in females. Conversely, in males, angiotensin receptor expression did not vary significantly with age. In conclusion, the angiotensin type 2 receptor modulates the chronic pressure-natriuresis relationship in an age and sex-dependent manner.

AB - Sex hormones regulate the renin angiotensin system. For example, estrogen enhances expression of the angiotensin type 2 receptor. We hypothesized that activation of the angiotensin type 2 receptor shifts the chronic pressure-natriuresis relationship leftward in females compared to males, and that this effect is lost with age. Mean arterial pressure was measured by radiotelemetry in adult (4 month old) and aged (14 month old) wild type and angiotensin type 2 receptor knockout male and female mice. Chronic pressure-natriuresis curves were constructed whilst mice were maintained on a normal salt (0.26 ) diet and following 6 days of high salt (5.0 ) diet. Mean arterial pressure was lower in adult wild type females than males (88+/-1 and 97+/-1 mmHg respectively), a difference that was maintained with age, but was absent in adult knockout mice. In wild-type females, the chronic pressure-natriuresis relationship was shifted leftward compared to knockout females, an effect that was lost with age. In males, the chronic pressure-natriuresis relationship was not influenced by angiotensin type 2 receptor deficiency. Compared to age-matched females, the chronic pressure-natriuresis relationships of male mice were shifted rightward. Renal expression of the angiotensin type 2 receptor was 4-fold greater in adult wild type females than males. With age, the angiotensin type 2 receptor to angiotensin type 1 receptor balance was reduced in females. Conversely, in males, angiotensin receptor expression did not vary significantly with age. In conclusion, the angiotensin type 2 receptor modulates the chronic pressure-natriuresis relationship in an age and sex-dependent manner.

UR - http://ajprenal.physiology.org/content/307/8/F901.full-text.pdf+html

U2 - 10.1152/ajprenal.00288.2014

DO - 10.1152/ajprenal.00288.2014

M3 - Article

VL - 307

SP - 901

EP - 907

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 1522-1466

IS - 8

ER -