Serum leukotriene B4 and hydroxyeicosatetraenoic acid in the prediction of pre-eclampsia

Leilani L. Santos, Dagmar Wertaschnigg, Daniel L. Rolnik, Fabricio Da Silva Costa, Argyro Syngelaki, Evdokia Dimitriadis, Kypros H. Nicolaides

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4 Citations (Scopus)


Introduction: Pre-eclampsia (PE) affects 2–8% of pregnancies worldwide. Despite identification of numerous possible biomarkers, accurate prediction and early diagnosis of PE remain challenging. We examined the potential of leukotriene B4 (LTB4) and 15-hydroxyeicosatetraenoic acid (15(S)-HETE) as biomarkers of PE by comparing serum levels at three gestational age (GA) groups between normotensive pregnancies and asymptomatic women who subsequently developed preterm or term-PE. Methods: This is a case-control study drawn from a prospective study of adverse pregnancy outcomes with serum samples collected at 19–24 weeks (n = 48), 30–34 weeks (n = 101) and 35–37 weeks (n = 54) GA. LTB4 and 15(S)-HETE levels were determined by ELISA. Serum level multiples of the median (MoM) were compared between normal and PE-pregnancies. Association between LTB4 and 15(S)-HETE and GA at delivery was investigated with Cox proportional-hazards models. Results: Serum LTB4 levels were lower in women of East-Asian ethnicity, higher in women with PE history, and increased with GA in normotensive pregnancies, but not in PE. LTB4 was elevated at 19–24 weeks in women who developed preterm-PE. There was a negative association between LTB4 MoM and interval between sampling and delivery with PE at 19–24 weeks only. Serum 15(S)-HETE levels were not influenced by GA at testing and were elevated in women of South-Asian ethnicity. Median 15(S)-HETE levels were unchanged in preterm and term-PE at any GA. Discussion: LTB4 was higher at 19–24 weeks in pregnancies that developed preterm-PE versus unaffected pregnancies, suggesting it is a potentially useful predictive marker of preterm PE in the second trimester.

Original languageEnglish
Pages (from-to)76-81
Number of pages6
Publication statusPublished - 1 Jan 2021


  • 15(S)-HETE
  • Biomarker
  • Eicosanoids
  • Leukotriene
  • LTB4
  • Pre-eclampsia

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