Sertoli cell differentiation and gonadogenesis in Alligator mississippiensis

Craig A. Smith, Jean M.P. Joss

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

Temperature‐dependent sex determination (TSD) was first described for a crocodilian in Alligator mississippiensis, in which egg incubation at 33°C produces 100% male hatchlings and incubation at 30°C produces 100% females. Ultrastructural signs of testis differentiation in embryos incubated at 33°C are first detected during days 28–34 (developmental stages 21–22), when small numbers of enlarged “presumptive” Sertoli cells (pre‐Sertoli cells) appear in the gonadal medulla of most embryos. These pre‐Sertoli cells proliferate and differentiate into Sertoli cells during stage 23 at 33°C (days 35–41), marking seminiferous cord formation. At 30°C, some pre‐Sertoli‐like cells are also present in the medulla, but they are less numerous than at 33°C and they do not differentiate into Sertoli cells. Ovary differentiation at 30°C is characterized by proliferation of cortical germs cells from day 39 (stage 22), closely followed by proliferation of somatic (pre‐follicular) cells in the cortex and regression of the medullary cords from day 43 (stage 23). We hypothesize that TSD in the alligator might be a function of a temperature‐derived mismatch between Sertoli cell differentiation and germ cell/cortex proliferation. © 1994 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)57-70
Number of pages14
JournalJournal of Experimental Zoology
Volume270
Issue number1
DOIs
Publication statusPublished - 1 Jan 1994
Externally publishedYes

Cite this

@article{ef2b78e9aa7d4d30b85e15077c97e218,
title = "Sertoli cell differentiation and gonadogenesis in Alligator mississippiensis",
abstract = "Temperature‐dependent sex determination (TSD) was first described for a crocodilian in Alligator mississippiensis, in which egg incubation at 33°C produces 100{\%} male hatchlings and incubation at 30°C produces 100{\%} females. Ultrastructural signs of testis differentiation in embryos incubated at 33°C are first detected during days 28–34 (developmental stages 21–22), when small numbers of enlarged “presumptive” Sertoli cells (pre‐Sertoli cells) appear in the gonadal medulla of most embryos. These pre‐Sertoli cells proliferate and differentiate into Sertoli cells during stage 23 at 33°C (days 35–41), marking seminiferous cord formation. At 30°C, some pre‐Sertoli‐like cells are also present in the medulla, but they are less numerous than at 33°C and they do not differentiate into Sertoli cells. Ovary differentiation at 30°C is characterized by proliferation of cortical germs cells from day 39 (stage 22), closely followed by proliferation of somatic (pre‐follicular) cells in the cortex and regression of the medullary cords from day 43 (stage 23). We hypothesize that TSD in the alligator might be a function of a temperature‐derived mismatch between Sertoli cell differentiation and germ cell/cortex proliferation. {\circledC} 1994 Wiley‐Liss, Inc.",
author = "Smith, {Craig A.} and Joss, {Jean M.P.}",
year = "1994",
month = "1",
day = "1",
doi = "10.1002/jez.1402700107",
language = "English",
volume = "270",
pages = "57--70",
journal = "Journal of Experimental Zoology",
issn = "0022-104X",
publisher = "Wiley-Blackwell",
number = "1",

}

Sertoli cell differentiation and gonadogenesis in Alligator mississippiensis. / Smith, Craig A.; Joss, Jean M.P.

In: Journal of Experimental Zoology, Vol. 270, No. 1, 01.01.1994, p. 57-70.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Sertoli cell differentiation and gonadogenesis in Alligator mississippiensis

AU - Smith, Craig A.

AU - Joss, Jean M.P.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Temperature‐dependent sex determination (TSD) was first described for a crocodilian in Alligator mississippiensis, in which egg incubation at 33°C produces 100% male hatchlings and incubation at 30°C produces 100% females. Ultrastructural signs of testis differentiation in embryos incubated at 33°C are first detected during days 28–34 (developmental stages 21–22), when small numbers of enlarged “presumptive” Sertoli cells (pre‐Sertoli cells) appear in the gonadal medulla of most embryos. These pre‐Sertoli cells proliferate and differentiate into Sertoli cells during stage 23 at 33°C (days 35–41), marking seminiferous cord formation. At 30°C, some pre‐Sertoli‐like cells are also present in the medulla, but they are less numerous than at 33°C and they do not differentiate into Sertoli cells. Ovary differentiation at 30°C is characterized by proliferation of cortical germs cells from day 39 (stage 22), closely followed by proliferation of somatic (pre‐follicular) cells in the cortex and regression of the medullary cords from day 43 (stage 23). We hypothesize that TSD in the alligator might be a function of a temperature‐derived mismatch between Sertoli cell differentiation and germ cell/cortex proliferation. © 1994 Wiley‐Liss, Inc.

AB - Temperature‐dependent sex determination (TSD) was first described for a crocodilian in Alligator mississippiensis, in which egg incubation at 33°C produces 100% male hatchlings and incubation at 30°C produces 100% females. Ultrastructural signs of testis differentiation in embryos incubated at 33°C are first detected during days 28–34 (developmental stages 21–22), when small numbers of enlarged “presumptive” Sertoli cells (pre‐Sertoli cells) appear in the gonadal medulla of most embryos. These pre‐Sertoli cells proliferate and differentiate into Sertoli cells during stage 23 at 33°C (days 35–41), marking seminiferous cord formation. At 30°C, some pre‐Sertoli‐like cells are also present in the medulla, but they are less numerous than at 33°C and they do not differentiate into Sertoli cells. Ovary differentiation at 30°C is characterized by proliferation of cortical germs cells from day 39 (stage 22), closely followed by proliferation of somatic (pre‐follicular) cells in the cortex and regression of the medullary cords from day 43 (stage 23). We hypothesize that TSD in the alligator might be a function of a temperature‐derived mismatch between Sertoli cell differentiation and germ cell/cortex proliferation. © 1994 Wiley‐Liss, Inc.

UR - http://www.scopus.com/inward/record.url?scp=84993899437&partnerID=8YFLogxK

U2 - 10.1002/jez.1402700107

DO - 10.1002/jez.1402700107

M3 - Article

VL - 270

SP - 57

EP - 70

JO - Journal of Experimental Zoology

JF - Journal of Experimental Zoology

SN - 0022-104X

IS - 1

ER -