Serpins and the complement system

Laszlo Beinrohr, Thomas Murray-Rust, Leanne Dyksterhuis, Peter Zavodszky, Peter Gal, Robert Pike, Lakshmi Wijeyewickrema

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

10 Citations (Scopus)

Abstract

C1-inhibitor (serpin G1) is a 105kDa inhibitor which functions as a major antiinflammatory protein in the body. It has its effects via inhibition of the proteases of the complement system and contact system of coagulation, as well as several direct effects mediated by its unique highly glycosylated N-terminal domain. The serpin controls a number of different proteases very efficiently and for some of these the function is augmented by the cofactor, heparin. Here, we describe the preparation of human plasma and recombinant C1-inhibitor and the basic methods required for their characterization, using the complement enzyme C1s as an example of a target enzyme.
Original languageEnglish
Title of host publicationMethods in Enzymology, Volume 499: Biology of Serpins
EditorsJames C Whisstock, Phillip I Bird
Place of PublicationUK
PublisherAcademic Press
Pages55 - 75
Number of pages21
Edition1st
ISBN (Print)9780123864710
DOIs
Publication statusPublished - 2011

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