Serpinb9 is a marker of antigen cross-presenting dendritic cells

M.S. Mangan, J. Vega-Ramos, L.T. Joeckel, A.J. Mitchell, A. Rizzitelli, B. Roediger, D. Kaiserman, W.W. Weninger, J.A. Villadangos, P.I. Bird

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Serpinb9 (Sb9, also called Spi6) is an intracellular inhibitor of granzyme B (grB) that protects cytotoxic lymphocytes from grB-mediated death. In addition, Sb9 is also expressed in accessory immune cells, including dendritic cells (DCs), although its role is debated. Recently, we have demonstrated that Sb9 plays a grB-independent role in cross-presentation of antigens by CD8+ DCs. Here, using a mouse line expressing green fluorescent protein knocked in under the control of the Sb9 promoter, we demonstrate that Sb9 expression is highest in those tissue-resident and migratory DC subsets capable of cross-presentation. Further, we show that CD8+ DCs can be divided into two subsets based on Sb9 expression, and that only the subset expressing higher levels of Sb9 is capable of cross-presentation. These findings add support for role for Sb9 cross-presentation, and indicate that high Sb9 expression is a novel marker of cross-presentation capable DCs.

Original languageEnglish
Pages (from-to)50-56
Number of pages7
JournalMolecular Immunology
Volume82
DOIs
Publication statusPublished - Feb 2017

Keywords

  • Cross-presentation
  • DCs
  • Dendritic cell
  • Granzyme B
  • Serpinb9
  • SPI6

Cite this

Mangan, M. S., Vega-Ramos, J., Joeckel, L. T., Mitchell, A. J., Rizzitelli, A., Roediger, B., ... Bird, P. I. (2017). Serpinb9 is a marker of antigen cross-presenting dendritic cells. Molecular Immunology, 82, 50-56. https://doi.org/10.1016/j.molimm.2016.12.011
Mangan, M.S. ; Vega-Ramos, J. ; Joeckel, L.T. ; Mitchell, A.J. ; Rizzitelli, A. ; Roediger, B. ; Kaiserman, D. ; Weninger, W.W. ; Villadangos, J.A. ; Bird, P.I. / Serpinb9 is a marker of antigen cross-presenting dendritic cells. In: Molecular Immunology. 2017 ; Vol. 82. pp. 50-56.
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Mangan, MS, Vega-Ramos, J, Joeckel, LT, Mitchell, AJ, Rizzitelli, A, Roediger, B, Kaiserman, D, Weninger, WW, Villadangos, JA & Bird, PI 2017, 'Serpinb9 is a marker of antigen cross-presenting dendritic cells' Molecular Immunology, vol. 82, pp. 50-56. https://doi.org/10.1016/j.molimm.2016.12.011

Serpinb9 is a marker of antigen cross-presenting dendritic cells. / Mangan, M.S.; Vega-Ramos, J.; Joeckel, L.T.; Mitchell, A.J.; Rizzitelli, A.; Roediger, B.; Kaiserman, D.; Weninger, W.W.; Villadangos, J.A.; Bird, P.I.

In: Molecular Immunology, Vol. 82, 02.2017, p. 50-56.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Mangan, M.S.

AU - Vega-Ramos, J.

AU - Joeckel, L.T.

AU - Mitchell, A.J.

AU - Rizzitelli, A.

AU - Roediger, B.

AU - Kaiserman, D.

AU - Weninger, W.W.

AU - Villadangos, J.A.

AU - Bird, P.I.

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Mangan MS, Vega-Ramos J, Joeckel LT, Mitchell AJ, Rizzitelli A, Roediger B et al. Serpinb9 is a marker of antigen cross-presenting dendritic cells. Molecular Immunology. 2017 Feb;82:50-56. https://doi.org/10.1016/j.molimm.2016.12.011