Serpinb9 is a marker of antigen cross-presenting dendritic cells

M.S. Mangan, J. Vega-Ramos, L.T. Joeckel, A.J. Mitchell, A. Rizzitelli, B. Roediger, D. Kaiserman, W.W. Weninger, J.A. Villadangos, P.I. Bird

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Serpinb9 (Sb9, also called Spi6) is an intracellular inhibitor of granzyme B (grB) that protects cytotoxic lymphocytes from grB-mediated death. In addition, Sb9 is also expressed in accessory immune cells, including dendritic cells (DCs), although its role is debated. Recently, we have demonstrated that Sb9 plays a grB-independent role in cross-presentation of antigens by CD8+ DCs. Here, using a mouse line expressing green fluorescent protein knocked in under the control of the Sb9 promoter, we demonstrate that Sb9 expression is highest in those tissue-resident and migratory DC subsets capable of cross-presentation. Further, we show that CD8+ DCs can be divided into two subsets based on Sb9 expression, and that only the subset expressing higher levels of Sb9 is capable of cross-presentation. These findings add support for role for Sb9 cross-presentation, and indicate that high Sb9 expression is a novel marker of cross-presentation capable DCs.

Original languageEnglish
Pages (from-to)50-56
Number of pages7
JournalMolecular Immunology
Volume82
DOIs
Publication statusPublished - Feb 2017

Keywords

  • Cross-presentation
  • DCs
  • Dendritic cell
  • Granzyme B
  • Serpinb9
  • SPI6

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