SerpinB2 deficiency modulates Th1Th2 responses after schistosome infection

Wayne Schroder, Joy Gardner, T Le, Mary Duke, Melissa Burke, Malcolm Jones, Donald McManus, Andreas Suhrbier

Research output: Contribution to journalArticleResearchpeer-review

34 Citations (Scopus)

Abstract

SerpinB2, also known as plasminogen activator inhibitor type-2, is a major product of macrophages and is upregulated during many infections. Although SerpinB2 inhibits urokinase plasminogen activator in vitro, evidence that this represents its physiological role in vivo is not compelling. We have recently shown that SerpinB2-/-mice generate enhanced Th1 responses after immunization with a Th1 immunogen. Herein,we show that Schistosoma japonicum granulomas induced liver SerpinB2 mRNA expression by >600-fold in wild-type mice. In SerpinB2-/- mice, worm and egg burden, and granuloma number and volume were unaffected. However, granulomas in these mice were associated with reduced fibrosis (as determined by Sirius red staining and image analysis) and increased iNOS, IL-6, IL-10 and TNFa and decreased Arg 1 and IL-13 mRNA expression. SerpinB2-/- mice immunized with soluble egg antigen (SEA) also showed reduced levels of SEA-specific IgG1. SerpinB2 deficiency thus promoted certain Th1 and reduced certain Th2 responses in response to this Th2 immunogen.
Original languageEnglish
Pages (from-to)764 - 768
Number of pages5
JournalParasite Immunology
Volume32
Issue number11-12
DOIs
Publication statusPublished - 2010
Externally publishedYes

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