Sequencing B cell receptors from ferrets (Mustela putorius furo)

Julius Wong, Celeste M. Tai, Aeron C. Hurt, Hyon Xhi Tan, Stephen J. Kent, Adam K. Wheatley

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

The domestic ferret (Mustela putorius furo) provides a critical animal model to study human respiratory diseases. However immunological insights are restricted due to a lack of ferretspecific reagents and limited genetic information about ferret B and T cell receptors. Here, variable, diversity and joining genes within the ferret kappa, lambda and heavy chain immunoglobulin loci were annotated using available genomic information. A multiplex PCR approach was derived that facilitated the recovery of paired heavy and light chain immunoglobulin sequences from single sorted ferret B cells, allowing validation of predicted germline gene sequences and the identification of putative novel germlines. Eukaryotic expression vectors were developed that enabled the generation of recombinant ferret monoclonal antibodies. This work advances the ferret as an informative immunological model for viral diseases by allowing the in-depth interrogation of antibody-based immunity.

Original languageEnglish
Article numbere0233794
Number of pages18
JournalPLoS ONE
Volume15
Issue number5
DOIs
Publication statusPublished - 29 May 2020

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