Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor

Angel Alberto Jesus Torriero, Eloy Salinas, Julio Raba, Juana J Silber

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41 Citations (Scopus)

Abstract

The high sensitivity that can be attained using an enzymic system and mediated by catechol has been verified by online interfacing of a rotating biosensor and continuous-flow/stopped-flow/continuous-flow processing. Horseradish peroxidase, HRP, [EC 1.11.1.7], immobilized on a rotating disk, in the presence of hydrogen peroxide catalyzed the oxidn. of catechol, whose back electrochem. redn. was detected on glassy carbon electrode surface at -200 mV. Thus, when ciprofloxacin (CF) or norfloxacin (NF) was added to the soln., these piperazine-contg. compds. participate in Michael addn. reactions with catechol to form the corresponding aminoquinone deriv., decreasing the peak current obtained in proportion with the increase of its concn. CF was used as the model piperazine-contg. compd. for the study. The influence of indicator compn. on the nature of the anal. response has been assessed through examg. the electrochem. properties of three derivs. Interference by electroactive species (ascorbate, urate, and tyrosine) and other physiol. constituents (cysteine, glutathione) has also been assessed. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)109 - 115
Number of pages7
JournalBiosensors and Bioelectronics
Volume22
Issue number1
Publication statusPublished - 2006
Externally publishedYes

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