TY - JOUR
T1 - Semliki Forest virus as an expression vector in insect cell lines
AU - Pettigrew, M
AU - O'Neill, Scott Leslie
PY - 1999
Y1 - 1999
N2 - Studies were undertaken to determine if replication-deficient Semliki Forest virus expression vectors could be successfully used to express foreign gene constructs in insect cell lines. Using green fluorescent protein (GFP) as a marker we recorded infection levers of nearly 100 in the Aedes albopictus cell lines C6/36 and Aa23T, as well as in the Ae. aegypti cell line MOS20. The virus was capable of infecting an Anopheles gambiae cell line MOS55. The amount of GFP protein produced in each cell line was quantified, Northern analysis of viral transcription revealed the presence of novel transcripts in Aa23T, C6/36, and MOS55 cell lines, but not in the BHK or MOS20, The initial characterization of these transcripts is described.
AB - Studies were undertaken to determine if replication-deficient Semliki Forest virus expression vectors could be successfully used to express foreign gene constructs in insect cell lines. Using green fluorescent protein (GFP) as a marker we recorded infection levers of nearly 100 in the Aedes albopictus cell lines C6/36 and Aa23T, as well as in the Ae. aegypti cell line MOS20. The virus was capable of infecting an Anopheles gambiae cell line MOS55. The amount of GFP protein produced in each cell line was quantified, Northern analysis of viral transcription revealed the presence of novel transcripts in Aa23T, C6/36, and MOS55 cell lines, but not in the BHK or MOS20, The initial characterization of these transcripts is described.
UR - http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2583.1999.83132.x/pdf
U2 - 10.1046/j.1365-2583.1999.83132.x
DO - 10.1046/j.1365-2583.1999.83132.x
M3 - Article
VL - 8
SP - 409
EP - 414
JO - Insect Molecular Biology
JF - Insect Molecular Biology
SN - 0962-1075
IS - 3
ER -