Selectivity optimisation of biomimetic molecularly imprinted polymer thin films

Reinhard I Boysen, Shuyan Li, Jamil Chowdhury, Lachlan Schwarz, Milton Thomas William Hearn

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This study describes the design and synthesis of molecularly imprinted polymers (MIPs) as patterned thin films, based on molecular modelling of functional monomer-template interactions and validation by NMR-spectroscopy. MIPs were prepared by dissolving the specific template and functional methacrylate monomer at varying ratios with a cross-linker and initiator in a porogenic solvent, spin-coating the solution onto 3-(trimethoxysilyl)propyl methacrylate-functionalised silicon wafers and photo-polymerisation. After template extraction, the film thickness and topography were characterised with atomic force microscopy. A 4-vinylpyridine-MIP thin film layer was then deposited by photo-lithographic etching onto this polymerised methacrylic acid MIP thin film, resulting in a grid-patterned surface in which two different MIPs alternated at a micron scale. Selectivity differences towards fluorescent template analogues were documented using fluorescence microscopy. This side-by-side comparison on the same thin film allows fast and cost-effective assessment of MIP selectivities with various biomolecules.
Original languageEnglish
Pages (from-to)81 - 84
Number of pages4
JournalMicroelectronic Engineering
Volume97
DOIs
Publication statusPublished - 2012

Cite this

Boysen, Reinhard I ; Li, Shuyan ; Chowdhury, Jamil ; Schwarz, Lachlan ; Hearn, Milton Thomas William. / Selectivity optimisation of biomimetic molecularly imprinted polymer thin films. In: Microelectronic Engineering. 2012 ; Vol. 97. pp. 81 - 84.
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abstract = "This study describes the design and synthesis of molecularly imprinted polymers (MIPs) as patterned thin films, based on molecular modelling of functional monomer-template interactions and validation by NMR-spectroscopy. MIPs were prepared by dissolving the specific template and functional methacrylate monomer at varying ratios with a cross-linker and initiator in a porogenic solvent, spin-coating the solution onto 3-(trimethoxysilyl)propyl methacrylate-functionalised silicon wafers and photo-polymerisation. After template extraction, the film thickness and topography were characterised with atomic force microscopy. A 4-vinylpyridine-MIP thin film layer was then deposited by photo-lithographic etching onto this polymerised methacrylic acid MIP thin film, resulting in a grid-patterned surface in which two different MIPs alternated at a micron scale. Selectivity differences towards fluorescent template analogues were documented using fluorescence microscopy. This side-by-side comparison on the same thin film allows fast and cost-effective assessment of MIP selectivities with various biomolecules.",
author = "Boysen, {Reinhard I} and Shuyan Li and Jamil Chowdhury and Lachlan Schwarz and Hearn, {Milton Thomas William}",
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Selectivity optimisation of biomimetic molecularly imprinted polymer thin films. / Boysen, Reinhard I; Li, Shuyan; Chowdhury, Jamil; Schwarz, Lachlan; Hearn, Milton Thomas William.

In: Microelectronic Engineering, Vol. 97, 2012, p. 81 - 84.

Research output: Contribution to journalArticleResearchpeer-review

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AB - This study describes the design and synthesis of molecularly imprinted polymers (MIPs) as patterned thin films, based on molecular modelling of functional monomer-template interactions and validation by NMR-spectroscopy. MIPs were prepared by dissolving the specific template and functional methacrylate monomer at varying ratios with a cross-linker and initiator in a porogenic solvent, spin-coating the solution onto 3-(trimethoxysilyl)propyl methacrylate-functionalised silicon wafers and photo-polymerisation. After template extraction, the film thickness and topography were characterised with atomic force microscopy. A 4-vinylpyridine-MIP thin film layer was then deposited by photo-lithographic etching onto this polymerised methacrylic acid MIP thin film, resulting in a grid-patterned surface in which two different MIPs alternated at a micron scale. Selectivity differences towards fluorescent template analogues were documented using fluorescence microscopy. This side-by-side comparison on the same thin film allows fast and cost-effective assessment of MIP selectivities with various biomolecules.

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