Selective isolation of bacterial DNA from human clinical specimens

Hans Peter Horz, Sebastian Scheer, Frank Huenger, Morgana E. Vianna, Georg Conrads

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51 Citations (Scopus)

Abstract

We evaluated two DNA preparation strategies (MolYsis®, Molzym GmbH & Co. KG, Bremen, Germany) and Pureprove®, SIRS-Lab GmbH, Jena, Germany) to selectively extract bacterial DNA from human clinical samples. By testing 16 oral samples we found that human DNA could be largely eliminated while detectable levels of bacterial DNA were obtained with all samples. Both approaches hold great potential for microbial diagnostic systems.

Original languageEnglish
Pages (from-to)98-102
Number of pages5
JournalJournal of Microbiological Methods
Volume72
Issue number1
DOIs
Publication statusPublished - Jan 2008
Externally publishedYes

Keywords

  • Detection of human pathogens
  • Nucleic acid-based microbial diagnostic systems
  • Separation of human and bacterial DNA

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