Selective Distance Measurements Using Triple Spin Labeling with Gd3+, Mn2+, and a Nitroxide

Zuyan Wu, Akiva Feintuch, Alberto Collauto, Luke A. Adams, Luigi Aurelio, Bim Graham, Gottfried Otting, Daniella Goldfarb

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Distance measurements by pulse electron paramagnetic resonance techniques, such as double electron-electron resonance (DEER, also called PELDOR), have become an established tool to explore structural properties of biomacromolecules and their assemblies. In such measurements a pair of spin labels provides a single distance constraint. Here we show that by employing three different types of spin labels that differ in their spectroscopic and spin dynamics properties it is possible to extract three independent distances from a single sample. We demonstrate this using the Antennapedia homeodomain orthogonally labeled with Gd3+ and Mn2+ tags in complex with its cognate DNA binding site labeled with a nitroxide.

Original languageEnglish
Pages (from-to)5277-5282
Number of pages6
JournalJournal of Physical Chemistry Letters
Volume8
Issue number21
DOIs
Publication statusPublished - 2 Nov 2017

Cite this

Wu, Zuyan ; Feintuch, Akiva ; Collauto, Alberto ; Adams, Luke A. ; Aurelio, Luigi ; Graham, Bim ; Otting, Gottfried ; Goldfarb, Daniella. / Selective Distance Measurements Using Triple Spin Labeling with Gd3+, Mn2+, and a Nitroxide. In: Journal of Physical Chemistry Letters. 2017 ; Vol. 8, No. 21. pp. 5277-5282.
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abstract = "Distance measurements by pulse electron paramagnetic resonance techniques, such as double electron-electron resonance (DEER, also called PELDOR), have become an established tool to explore structural properties of biomacromolecules and their assemblies. In such measurements a pair of spin labels provides a single distance constraint. Here we show that by employing three different types of spin labels that differ in their spectroscopic and spin dynamics properties it is possible to extract three independent distances from a single sample. We demonstrate this using the Antennapedia homeodomain orthogonally labeled with Gd3+ and Mn2+ tags in complex with its cognate DNA binding site labeled with a nitroxide.",
author = "Zuyan Wu and Akiva Feintuch and Alberto Collauto and Adams, {Luke A.} and Luigi Aurelio and Bim Graham and Gottfried Otting and Daniella Goldfarb",
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Wu, Z, Feintuch, A, Collauto, A, Adams, LA, Aurelio, L, Graham, B, Otting, G & Goldfarb, D 2017, 'Selective Distance Measurements Using Triple Spin Labeling with Gd3+, Mn2+, and a Nitroxide' Journal of Physical Chemistry Letters, vol. 8, no. 21, pp. 5277-5282. https://doi.org/10.1021/acs.jpclett.7b01739

Selective Distance Measurements Using Triple Spin Labeling with Gd3+, Mn2+, and a Nitroxide. / Wu, Zuyan; Feintuch, Akiva; Collauto, Alberto; Adams, Luke A.; Aurelio, Luigi; Graham, Bim; Otting, Gottfried; Goldfarb, Daniella.

In: Journal of Physical Chemistry Letters, Vol. 8, No. 21, 02.11.2017, p. 5277-5282.

Research output: Contribution to journalArticleResearchpeer-review

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