Selection of high-quality sperm with thousands of parallel channels

Mohammad Simchi, Jason Riordon, Jae Bem You, Yihe Wang, Sa Xiao, Alexander Lagunov, Thomas Hannam, Keith Jarvi, Reza Nosrati, David Sinton

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)


Sperm selection is essential for successful fertilization and embryo development. Current clinical sperm selection methods are labor-intensive and lack the selectivity required to isolate high-quality sperm. Microfluidic sperm selection approaches have shown promise but present a trade-off between the quality and quantity of selected sperm - clinicians demand both. The structure of the female reproductive tract helps to isolate a sufficient quantity of high-quality sperm for fertilization with densely folded epithelium that provides a multitude of longitudinally oriented pathways that guide sperm toward the fertilization site. Here, a three-dimensionally structured sperm selection device is presented that levers this highly parallelizedin vivomechanism forin vitrosperm selection. The device is inserted in a test tube atop 1 mL of raw semen and provides 6500 channels that isolate ∼100 000 high-DNA-integrity sperm for assisted reproduction. In side-by-side clinical testing, the developed approach outperforms the best current clinical methods by improving the DNA integrity of the selected sperm subpopulation up to 95%. Also, the device streamlines clinical workflow, reducing the time required for sperm preparation 3-fold. This single-tube, single-step sperm preparation approach promises to improve both the economics and outcomes of assisted reproduction practices, especially in cases with significant male-factors.

Original languageEnglish
Pages (from-to)2464-2475
Number of pages12
JournalLab on a Chip
Issue number12
Publication statusPublished - 13 May 2021

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