Abstract
Allergic asthma is responsible for widespread morbidity and mortality and its incidence has increased dramatically in industrialized countries over the past two decades. Here, we describe a new murine model of allergic asthma utilizing a novel allergen with intrinsic enzymatic activity similar to that reported for many environmental allergens. The allergen, NES, is excreted and secreted from the nematode Nippostrongylus brasiliensis, and can readily be isolated from in vitro parasite cultures. When NES is administered intranasally to presensitized mice, allergic airway disease develops, including airway hyper-responsiveness, airway eosinophilia, IgE antibody production and Th2 cytokine production. This disease is characteristic of atopic asthma and can be induced within 11 days, thus providing a platform for the rapid analysis of allergic disease and high throughput testing of immunomodulatory factors.
| Original language | English |
|---|---|
| Pages (from-to) | 40-47 |
| Number of pages | 8 |
| Journal | Immunology and Cell Biology |
| Volume | 83 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Feb 2005 |
| Externally published | Yes |
Keywords
- Allergen
- Asthma
- Nematode
- Nippostrongylus
- Th2
Cite this
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Secretory products from infective forms of Nippostrongylus brasiliensis induce a rapid allergic airway inflammatory response. / Marsland, Benjamin J.; Camberis, Mali; Le Gros, Graham.
In: Immunology and Cell Biology, Vol. 83, No. 1, 01.02.2005, p. 40-47.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Secretory products from infective forms of Nippostrongylus brasiliensis induce a rapid allergic airway inflammatory response
AU - Marsland, Benjamin J.
AU - Camberis, Mali
AU - Le Gros, Graham
PY - 2005/2/1
Y1 - 2005/2/1
N2 - Allergic asthma is responsible for widespread morbidity and mortality and its incidence has increased dramatically in industrialized countries over the past two decades. Here, we describe a new murine model of allergic asthma utilizing a novel allergen with intrinsic enzymatic activity similar to that reported for many environmental allergens. The allergen, NES, is excreted and secreted from the nematode Nippostrongylus brasiliensis, and can readily be isolated from in vitro parasite cultures. When NES is administered intranasally to presensitized mice, allergic airway disease develops, including airway hyper-responsiveness, airway eosinophilia, IgE antibody production and Th2 cytokine production. This disease is characteristic of atopic asthma and can be induced within 11 days, thus providing a platform for the rapid analysis of allergic disease and high throughput testing of immunomodulatory factors.
AB - Allergic asthma is responsible for widespread morbidity and mortality and its incidence has increased dramatically in industrialized countries over the past two decades. Here, we describe a new murine model of allergic asthma utilizing a novel allergen with intrinsic enzymatic activity similar to that reported for many environmental allergens. The allergen, NES, is excreted and secreted from the nematode Nippostrongylus brasiliensis, and can readily be isolated from in vitro parasite cultures. When NES is administered intranasally to presensitized mice, allergic airway disease develops, including airway hyper-responsiveness, airway eosinophilia, IgE antibody production and Th2 cytokine production. This disease is characteristic of atopic asthma and can be induced within 11 days, thus providing a platform for the rapid analysis of allergic disease and high throughput testing of immunomodulatory factors.
KW - Allergen
KW - Asthma
KW - Nematode
KW - Nippostrongylus
KW - Th2
UR - http://www.scopus.com/inward/record.url?scp=13144293063&partnerID=8YFLogxK
U2 - 10.1111/j.1440-1711.2004.01303.x
DO - 10.1111/j.1440-1711.2004.01303.x
M3 - Article
VL - 83
SP - 40
EP - 47
JO - Immunology and Cell Biology
JF - Immunology and Cell Biology
SN - 0818-9641
IS - 1
ER -