Secretion of apolipoprotein E from macrophages occurs via a protein kinase A- and calcium-dependent pathway along the microtubule network

Maaike Kockx, Dongni Guo, Thierry Huby, Philippe Lesnik, Jason Kay, Tharani Sabaretnam, Eve Jary, Michael Hill, Katharina Gaus, John Chapman, Jennifer Stow, Wendy Jessup, Leonard Kritharides

Research output: Contribution to journalArticleResearchpeer-review

26 Citations (Scopus)

Abstract

Macrophage-specific expression of apolipoprotein (apo)E protects against atherosclerosis; however, the signaling and trafficking pathways regulating secretion of apoE are unknown. We investigated the roles of the actin skeleton, microtubules, protein kinase A (PKA) and calcium (Ca2+) in regulating apoE secretion from macrophages. Disrupting microtubules with vinblastine or colchicine inhibited basal secretion of apoE substantially, whereas disruption of the actin skeleton had no effect. Structurally distinct inhibitors of PKA (H89, KT5720, inhibitory peptide PKI(14-22)) all decreased basal secretion of apoE by between 50 to 80 (P
Original languageEnglish
Pages (from-to)607 - 616
Number of pages10
JournalCirculation Research
Volume101
Issue number6
DOIs
Publication statusPublished - 2007
Externally publishedYes

Cite this