TY - JOUR
T1 - Secretion of apolipoprotein E from macrophages occurs via a protein kinase A- and calcium-dependent pathway along the microtubule network
AU - Kockx, Maaike
AU - Guo, Dongni
AU - Huby, Thierry
AU - Lesnik, Philippe
AU - Kay, Jason
AU - Sabaretnam, Tharani
AU - Jary, Eve
AU - Hill, Michael
AU - Gaus, Katharina
AU - Chapman, John
AU - Stow, Jennifer
AU - Jessup, Wendy
AU - Kritharides, Leonard
PY - 2007
Y1 - 2007
N2 - Macrophage-specific expression of apolipoprotein (apo)E protects against atherosclerosis; however, the signaling and trafficking pathways regulating secretion of apoE are unknown. We investigated the roles of the actin skeleton, microtubules, protein kinase A (PKA) and calcium (Ca2+) in regulating apoE secretion from macrophages. Disrupting microtubules with vinblastine or colchicine inhibited basal secretion of apoE substantially, whereas disruption of the actin skeleton had no effect. Structurally distinct inhibitors of PKA (H89, KT5720, inhibitory peptide PKI(14-22)) all decreased basal secretion of apoE by between 50 to 80 (P
AB - Macrophage-specific expression of apolipoprotein (apo)E protects against atherosclerosis; however, the signaling and trafficking pathways regulating secretion of apoE are unknown. We investigated the roles of the actin skeleton, microtubules, protein kinase A (PKA) and calcium (Ca2+) in regulating apoE secretion from macrophages. Disrupting microtubules with vinblastine or colchicine inhibited basal secretion of apoE substantially, whereas disruption of the actin skeleton had no effect. Structurally distinct inhibitors of PKA (H89, KT5720, inhibitory peptide PKI(14-22)) all decreased basal secretion of apoE by between 50 to 80 (P
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17660382
U2 - 10.1161/CIRCRESAHA.107.157198
DO - 10.1161/CIRCRESAHA.107.157198
M3 - Article
SN - 0009-7330
VL - 101
SP - 607
EP - 616
JO - Circulation Research
JF - Circulation Research
IS - 6
ER -