Secretion of apolipoprotein E from macrophages occurs via a protein kinase A- and calcium-dependent pathway along the microtubule network

Maaike Kockx; Dongni Guo; Thierry Huby; Philippe Lesnik; Jason Kay; Tharani Sabaretnam; Eve Jary; Michael Hill; Katharina Gaus; John Chapman; Jennifer Stow; Wendy Jessup; Leonard Kritharides

doi:10.1161/CIRCRESAHA.107.157198

Secretion of apolipoprotein E from macrophages occurs via a protein kinase A- and calcium-dependent pathway along the microtubule network

Maaike Kockx, Dongni Guo, Thierry Huby, Philippe Lesnik, Jason Kay, Tharani Sabaretnam, Eve Jary, Michael Hill, Katharina Gaus, John Chapman, Jennifer Stow, Wendy Jessup, Leonard Kritharides

Research output: Contribution to journal › Article › Research › peer-review

26 Citations (Scopus)

Abstract

Macrophage-specific expression of apolipoprotein (apo)E protects against atherosclerosis; however, the signaling and trafficking pathways regulating secretion of apoE are unknown. We investigated the roles of the actin skeleton, microtubules, protein kinase A (PKA) and calcium (Ca2+) in regulating apoE secretion from macrophages. Disrupting microtubules with vinblastine or colchicine inhibited basal secretion of apoE substantially, whereas disruption of the actin skeleton had no effect. Structurally distinct inhibitors of PKA (H89, KT5720, inhibitory peptide PKI(14-22)) all decreased basal secretion of apoE by between 50 to 80 (P

Original language	English
Pages (from-to)	607 - 616
Number of pages	10
Journal	Circulation Research
Volume	101
Issue number	6
DOIs	https://doi.org/10.1161/CIRCRESAHA.107.157198
Publication status	Published - 2007
Externally published	Yes

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10.1161/CIRCRESAHA.107.157198

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Kockx, M., Guo, D., Huby, T., Lesnik, P., Kay, J., Sabaretnam, T., Jary, E., Hill, M., Gaus, K., Chapman, J., Stow, J., Jessup, W., & Kritharides, L. (2007). Secretion of apolipoprotein E from macrophages occurs via a protein kinase A- and calcium-dependent pathway along the microtubule network. Circulation Research, 101(6), 607 - 616. https://doi.org/10.1161/CIRCRESAHA.107.157198

Kockx, Maaike ; Guo, Dongni ; Huby, Thierry ; Lesnik, Philippe ; Kay, Jason ; Sabaretnam, Tharani ; Jary, Eve ; Hill, Michael ; Gaus, Katharina ; Chapman, John ; Stow, Jennifer ; Jessup, Wendy ; Kritharides, Leonard. / Secretion of apolipoprotein E from macrophages occurs via a protein kinase A- and calcium-dependent pathway along the microtubule network. In: Circulation Research. 2007 ; Vol. 101, No. 6. pp. 607 - 616.

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title = "Secretion of apolipoprotein E from macrophages occurs via a protein kinase A- and calcium-dependent pathway along the microtubule network",

abstract = "Macrophage-specific expression of apolipoprotein (apo)E protects against atherosclerosis; however, the signaling and trafficking pathways regulating secretion of apoE are unknown. We investigated the roles of the actin skeleton, microtubules, protein kinase A (PKA) and calcium (Ca2+) in regulating apoE secretion from macrophages. Disrupting microtubules with vinblastine or colchicine inhibited basal secretion of apoE substantially, whereas disruption of the actin skeleton had no effect. Structurally distinct inhibitors of PKA (H89, KT5720, inhibitory peptide PKI(14-22)) all decreased basal secretion of apoE by between 50 to 80 (P",

author = "Maaike Kockx and Dongni Guo and Thierry Huby and Philippe Lesnik and Jason Kay and Tharani Sabaretnam and Eve Jary and Michael Hill and Katharina Gaus and John Chapman and Jennifer Stow and Wendy Jessup and Leonard Kritharides",

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Kockx, M, Guo, D, Huby, T, Lesnik, P, Kay, J, Sabaretnam, T, Jary, E, Hill, M, Gaus, K, Chapman, J, Stow, J, Jessup, W & Kritharides, L 2007, 'Secretion of apolipoprotein E from macrophages occurs via a protein kinase A- and calcium-dependent pathway along the microtubule network', Circulation Research, vol. 101, no. 6, pp. 607 - 616. https://doi.org/10.1161/CIRCRESAHA.107.157198

Secretion of apolipoprotein E from macrophages occurs via a protein kinase A- and calcium-dependent pathway along the microtubule network. / Kockx, Maaike; Guo, Dongni; Huby, Thierry; Lesnik, Philippe; Kay, Jason; Sabaretnam, Tharani; Jary, Eve; Hill, Michael; Gaus, Katharina; Chapman, John; Stow, Jennifer; Jessup, Wendy; Kritharides, Leonard.

In: Circulation Research, Vol. 101, No. 6, 2007, p. 607 - 616.

Research output: Contribution to journal › Article › Research › peer-review

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AU - Kay, Jason

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AU - Stow, Jennifer

AU - Jessup, Wendy

AU - Kritharides, Leonard

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