Second Messenger Assays for G Protein-Coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2

Karen J. Gregory; Patrick M. Sexton; Arthur Christopoulos; Caroline A. Hick

doi:10.1002/9780470749210.ch2

Second Messenger Assays for G Protein-Coupled Receptors: cAMP, Ca²⁺, Inositol Phosphates, ERK1/2

Karen J. Gregory, Patrick M. Sexton, Arthur Christopoulos, Caroline A. Hick

Drug Discovery Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

3 Citations (Scopus)

Original language	English
Title of host publication	G Protein-Coupled Receptors: Essential Methods
Publisher	Wiley-Blackwell
Pages	31-52
Number of pages	22
ISBN (Print)	9780470749142
DOIs	https://doi.org/10.1002/9780470749210.ch2
Publication status	Published - 25 Jan 2010

Keywords

Calcium ions - widely assayed intracellular messengers
CAMP, tightly regulated signalling molecule in extracellular signal transduction
G protein-coupled receptors (GPCRs)
Major intracellular signalling pathways from G protein coupling
Mitogen-activated protein kinases
Protocol 2.3 fluo-4 calcium assay
Second messenger assays for G protein-coupled receptors - cAMP, Ca2+, Inositol Phosphates, ERK1/2
Semi-automated filtration-based anion-exchange chromatography approaches
Successful assay - cells expressing GPCR of interest
Troubleshooting

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10.1002/9780470749210.ch2

Cite this

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title = "Second Messenger Assays for G Protein-Coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2",

keywords = "Calcium ions - widely assayed intracellular messengers, CAMP, tightly regulated signalling molecule in extracellular signal transduction, G protein-coupled receptors (GPCRs), Major intracellular signalling pathways from G protein coupling, Mitogen-activated protein kinases, Protocol 2.3 fluo-4 calcium assay, Second messenger assays for G protein-coupled receptors - cAMP, Ca2+, Inositol Phosphates, ERK1/2, Semi-automated filtration-based anion-exchange chromatography approaches, Successful assay - cells expressing GPCR of interest, Troubleshooting",

author = "Gregory, \{Karen J.\} and Sexton, \{Patrick M.\} and Arthur Christopoulos and Hick, \{Caroline A.\}",

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doi = "10.1002/9780470749210.ch2",

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AU - Gregory, Karen J.

AU - Sexton, Patrick M.

AU - Christopoulos, Arthur

AU - Hick, Caroline A.

PY - 2010/1/25

Y1 - 2010/1/25

KW - Calcium ions - widely assayed intracellular messengers

KW - CAMP, tightly regulated signalling molecule in extracellular signal transduction

KW - G protein-coupled receptors (GPCRs)

KW - Major intracellular signalling pathways from G protein coupling

KW - Mitogen-activated protein kinases

KW - Protocol 2.3 fluo-4 calcium assay

KW - Second messenger assays for G protein-coupled receptors - cAMP, Ca2+, Inositol Phosphates, ERK1/2

KW - Semi-automated filtration-based anion-exchange chromatography approaches

KW - Successful assay - cells expressing GPCR of interest

KW - Troubleshooting

UR - https://www.scopus.com/pages/publications/84886337050

U2 - 10.1002/9780470749210.ch2

DO - 10.1002/9780470749210.ch2

M3 - Chapter (Book)

AN - SCOPUS:84886337050

SN - 9780470749142

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EP - 52

BT - G Protein-Coupled Receptors: Essential Methods

PB - Wiley-Blackwell

ER -

Second Messenger Assays for G Protein-Coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2

Keywords

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Second Messenger Assays for G Protein-Coupled Receptors: cAMP, Ca²⁺, Inositol Phosphates, ERK1/2