Second Messenger Assays for G Protein-Coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2

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1 Citation (Scopus)
Original languageEnglish
Title of host publicationG Protein-Coupled Receptors: Essential Methods
Number of pages22
ISBN (Print)9780470749142
Publication statusPublished - 25 Jan 2010


  • Calcium ions - widely assayed intracellular messengers
  • CAMP, tightly regulated signalling molecule in extracellular signal transduction
  • G protein-coupled receptors (GPCRs)
  • Major intracellular signalling pathways from G protein coupling
  • Mitogen-activated protein kinases
  • Protocol 2.3 fluo-4 calcium assay
  • Second messenger assays for G protein-coupled receptors - cAMP, Ca2+, Inositol Phosphates, ERK1/2
  • Semi-automated filtration-based anion-exchange chromatography approaches
  • Successful assay - cells expressing GPCR of interest
  • Troubleshooting

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