Second Messenger Assays for G Protein-Coupled Receptors

cAMP, Ca2+, Inositol Phosphates, ERK1/2

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

1 Citation (Scopus)
Original languageEnglish
Title of host publicationG Protein-Coupled Receptors: Essential Methods
PublisherWiley-Blackwell
Pages31-52
Number of pages22
ISBN (Print)9780470749142
DOIs
Publication statusPublished - 25 Jan 2010

Keywords

  • Calcium ions - widely assayed intracellular messengers
  • CAMP, tightly regulated signalling molecule in extracellular signal transduction
  • G protein-coupled receptors (GPCRs)
  • Major intracellular signalling pathways from G protein coupling
  • Mitogen-activated protein kinases
  • Protocol 2.3 fluo-4 calcium assay
  • Second messenger assays for G protein-coupled receptors - cAMP, Ca2+, Inositol Phosphates, ERK1/2
  • Semi-automated filtration-based anion-exchange chromatography approaches
  • Successful assay - cells expressing GPCR of interest
  • Troubleshooting

Cite this

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title = "Second Messenger Assays for G Protein-Coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2",
keywords = "Calcium ions - widely assayed intracellular messengers, CAMP, tightly regulated signalling molecule in extracellular signal transduction, G protein-coupled receptors (GPCRs), Major intracellular signalling pathways from G protein coupling, Mitogen-activated protein kinases, Protocol 2.3 fluo-4 calcium assay, Second messenger assays for G protein-coupled receptors - cAMP, Ca2+, Inositol Phosphates, ERK1/2, Semi-automated filtration-based anion-exchange chromatography approaches, Successful assay - cells expressing GPCR of interest, Troubleshooting",
author = "Gregory, {Karen J.} and Sexton, {Patrick M.} and Arthur Christopoulos and Hick, {Caroline A.}",
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booktitle = "G Protein-Coupled Receptors: Essential Methods",
publisher = "Wiley-Blackwell",
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}

Second Messenger Assays for G Protein-Coupled Receptors : cAMP, Ca2+, Inositol Phosphates, ERK1/2. / Gregory, Karen J.; Sexton, Patrick M.; Christopoulos, Arthur; Hick, Caroline A.

G Protein-Coupled Receptors: Essential Methods. Wiley-Blackwell, 2010. p. 31-52.

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

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AU - Sexton, Patrick M.

AU - Christopoulos, Arthur

AU - Hick, Caroline A.

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KW - Protocol 2.3 fluo-4 calcium assay

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KW - Semi-automated filtration-based anion-exchange chromatography approaches

KW - Successful assay - cells expressing GPCR of interest

KW - Troubleshooting

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BT - G Protein-Coupled Receptors: Essential Methods

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