TY - JOUR
T1 - Second dimension column ensemble pressure tuning in comprehensive two-dimensional gas chromatography
AU - Sharif, Khan M
AU - Kulsing, Chadin
AU - Iris Da Silva Junior, Ademario
AU - Marriott, Philip
PY - 2018
Y1 - 2018
N2 - A pressure tunable (PT) coupled column ensemble has been implemented for the second dimension (2D) separation in comprehensive two dimensional gas chromatography (GC×PTGC). This process requires two columns to be connected by a pressure junction, as a replacement for a single narrow bore, short column in 2D. Various 2D1 and 2D2 columns may be selected to provide complementary selectivity (polarity) compared to the 1D column. The tunable residence time arising from differential pressure drop in each 2D column results in a tunable fractional contribution of each column in the 2D separation. A sample mixture comprising different chemical classes, including alkanes and alcohols, is used to identify the feasibility and extent of selectivity tuning possible in GC×PTGC. The column length is also varied due to the imposed challenge of wraparound in the PT coupled column system as pressures are adjusted in the 2D separation. Different experimental parameters, stationary phase materials and column lengths have been applied to investigate and understand the separation behaviour of the 2D PT coupled column GC×GC system. Results are discussed considering analyte retention time, peak width, linear velocity and the contribution of each 2D column. A specific and unexpected example of GC×GC separation was demonstrated where the peak positions of polar and apolar compounds could almost swap their 2D retention position by application of PT. Kerosene was analysed as an example of complex sample analysis by GC×PTGC system. This process is shown to be a practical approach for altering different stationary phase selectivities in a single 2D arrangement in GC×GC.
AB - A pressure tunable (PT) coupled column ensemble has been implemented for the second dimension (2D) separation in comprehensive two dimensional gas chromatography (GC×PTGC). This process requires two columns to be connected by a pressure junction, as a replacement for a single narrow bore, short column in 2D. Various 2D1 and 2D2 columns may be selected to provide complementary selectivity (polarity) compared to the 1D column. The tunable residence time arising from differential pressure drop in each 2D column results in a tunable fractional contribution of each column in the 2D separation. A sample mixture comprising different chemical classes, including alkanes and alcohols, is used to identify the feasibility and extent of selectivity tuning possible in GC×PTGC. The column length is also varied due to the imposed challenge of wraparound in the PT coupled column system as pressures are adjusted in the 2D separation. Different experimental parameters, stationary phase materials and column lengths have been applied to investigate and understand the separation behaviour of the 2D PT coupled column GC×GC system. Results are discussed considering analyte retention time, peak width, linear velocity and the contribution of each 2D column. A specific and unexpected example of GC×GC separation was demonstrated where the peak positions of polar and apolar compounds could almost swap their 2D retention position by application of PT. Kerosene was analysed as an example of complex sample analysis by GC×PTGC system. This process is shown to be a practical approach for altering different stationary phase selectivities in a single 2D arrangement in GC×GC.
KW - Comprehensive two dimensional gas chromatography
KW - Coupled columns
KW - Pressure tuning
KW - Second dimension selectivity tuning
UR - http://www.scopus.com/inward/record.url?scp=85032976571&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2017.10.060
DO - 10.1016/j.chroma.2017.10.060
M3 - Article
AN - SCOPUS:85032976571
VL - 1536
SP - 39
EP - 49
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
ER -