Screening food-based libraries to identify antihypertensive bioactives

Paul F. Rogers, G S Patten, Louise Elizabeth Bennett, Mahinda Y. Abeywardena

Research output: Contribution to conferenceAbstract

Abstract

Background - Development of functional foods and nutraceuticals targeted to promote cardiovascular benefits is anactive area of research at present. Supporting evidence exists for several such bioactives including plant sterols, n-3 polyunsaturated fatty acids, polyphenols and peptides from various sources.

Objective - To develop and validate suitable assay systems to allow rapid screening of different food based peptide libraries in an attempt to identify potential bioactives that may possess antihypertensive properties.

Design - A rapid spectrophotometric assay to identify inhibitors of angiotensin-converting enzyme (ACE) was developed based on the method of Cushman and Cheung. Other potential target mechanisms identified include scavenging of free radicals and inhibition of the action of angiotensin II at its receptor.

Outcomes - A reproducible assay for ACE activity (CV 4.1%) was established and validated using the pharmacological inhibitor captopril, and Val-Ala-Pro, a known inhibitory tri-peptide, as standards. A selection of commercially available, vegetable derived protein hydrolysates demonstrated ACE inhibitory activity (wheat, 20-50%; soy, up to 20%; rice, 10-70% and pea, 10-60%). In some cases it was possible to enhance this activity by further fractionation.

Conclusions - The ability of some food-based fractions to inhibit ACE was demonstrated. By targeting different mechanisms of antihypertensive action, the potential exists to improve the efficacy of dietary interventions for lowering blood pressure.
Original languageEnglish
Number of pages1
Publication statusPublished - 2005
Externally publishedYes

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