Screening and characterization of squalene-producing thraustochytrids from Hong Kong mangroves

Qian Li, Guan Qun Chen, King Wai Fan, F. U.Ping Lu, Tsunehiro Aki, Yue Jiang

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45 Citations (Scopus)

Abstract

Eighteen strains of thraustochytrids were newly isolated from Hong Kong mangroves, and their fatty acid and squalene contents were analyzed. All strains could grow well heterotrophically with glucose as the sole carbon source. All of them had the typical fatty acid profile of thraustchytrids and could produce a large amount of docosahexaenoic acid. The cell dry weight ranged from 5.49 to 15.62 g/L and squalene content from 0.02 to 0.18 mg/g at 72 h. The highest squalene-producing strain, BR-MP4-A1 was identified as a new strain of Aurantiochytrium species through sequence comparison of the 18S rRNA gene. The highest biomass of Aurantiochytrium sp. BR-MP4-A1 was achieved at 72 h, whereas its squalene content reached the maximum of 0.567 mg/g at 36 h but decreased rapidly thereafter. The production of squalene by thraustochytrids might be highly influenced by culture conditions.

Original languageEnglish
Pages (from-to)4267-4272
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number10
DOIs
Publication statusPublished - 27 May 2009
Externally publishedYes

Keywords

  • Aurantiochytrium sp
  • Phylogenetic identification
  • Squalene
  • Thraustochytrid

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