Schistosoma japonicum

Monoclonal antibodies to the Mr 26,000 schistosome glutathione S-transferase (Sj26) in an assay for circulating antigen in infected individuals

K. M. Davern, W. U. Tiu, N. Samaras, D. P. Gearing, B. E. Hall, E. G. Garcia, G. F. Mitchell

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

Two monoclonal antibodies have been produced that bind to separate epitopes on the Mr 26,000 glutathione S-transferase (GST) of Schistosoma japonicum worms (Sj26). Both antibodies have been used in an enzyme immunoassay (EIA) with sera from infected individuals from the Philippines. Relatively high signals were obtained with sera from some, but not all, individuals who are positive for fecal eggs. Evidence was obtained that the material detected by the monoclonal antibodies was present in minute amounts and in some sera was bound in a complex with phosphorylcholinecontaining molecules. It could not be absorbed by reaction with glutathioneagarose columns. There was no detectable immunoglobulin in the complex. The possibility exists that the complexes are composed of schistosome GST, or fragments, and damaged tegumental lipids shed as a result of surface immune attack. However, the presence of the native Sj26 molecule has not been proven. More detailed longitudinal studies in endemic areas are required to determine whether the assay can be used as an indicator of acquired resistance ("concomitant immunity") and whether it will be useful in the search for immunological correlates of this resistance in humans.

Original languageEnglish
Pages (from-to)293-304
Number of pages12
JournalExperimental Parasitology
Volume70
Issue number3
DOIs
Publication statusPublished - 1 Jan 1990

Keywords

  • Schistosoma japonicum
  • Trematode

Cite this

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title = "Schistosoma japonicum: Monoclonal antibodies to the Mr 26,000 schistosome glutathione S-transferase (Sj26) in an assay for circulating antigen in infected individuals",
abstract = "Two monoclonal antibodies have been produced that bind to separate epitopes on the Mr 26,000 glutathione S-transferase (GST) of Schistosoma japonicum worms (Sj26). Both antibodies have been used in an enzyme immunoassay (EIA) with sera from infected individuals from the Philippines. Relatively high signals were obtained with sera from some, but not all, individuals who are positive for fecal eggs. Evidence was obtained that the material detected by the monoclonal antibodies was present in minute amounts and in some sera was bound in a complex with phosphorylcholinecontaining molecules. It could not be absorbed by reaction with glutathioneagarose columns. There was no detectable immunoglobulin in the complex. The possibility exists that the complexes are composed of schistosome GST, or fragments, and damaged tegumental lipids shed as a result of surface immune attack. However, the presence of the native Sj26 molecule has not been proven. More detailed longitudinal studies in endemic areas are required to determine whether the assay can be used as an indicator of acquired resistance ({"}concomitant immunity{"}) and whether it will be useful in the search for immunological correlates of this resistance in humans.",
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Schistosoma japonicum : Monoclonal antibodies to the Mr 26,000 schistosome glutathione S-transferase (Sj26) in an assay for circulating antigen in infected individuals. / Davern, K. M.; Tiu, W. U.; Samaras, N.; Gearing, D. P.; Hall, B. E.; Garcia, E. G.; Mitchell, G. F.

In: Experimental Parasitology, Vol. 70, No. 3, 01.01.1990, p. 293-304.

Research output: Contribution to journalArticleResearchpeer-review

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T2 - Monoclonal antibodies to the Mr 26,000 schistosome glutathione S-transferase (Sj26) in an assay for circulating antigen in infected individuals

AU - Davern, K. M.

AU - Tiu, W. U.

AU - Samaras, N.

AU - Gearing, D. P.

AU - Hall, B. E.

AU - Garcia, E. G.

AU - Mitchell, G. F.

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Y1 - 1990/1/1

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