Sample loading in gel electrophoresis using adapted 3D printers

Tao Tao; Alexey Kopyl; Yuki Yew; Fawaz El-Dani; Hassan Ali Abid; Thomas Hiscox; Oi Wah Liew; Tuck Wah Ng

doi:10.1016/j.ab.2024.115721

Sample loading in gel electrophoresis using adapted 3D printers

Tao Tao, Alexey Kopyl, Yuki Yew, Fawaz El-Dani, Hassan Ali Abid, Thomas Hiscox, Oi Wah Liew, Tuck Wah Ng

Research output: Contribution to journal › Article › Research › peer-review

Abstract

In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.

Original language	English
Article number	115721
Number of pages	3
Journal	Analytical Biochemistry
Volume	697
DOIs	https://doi.org/10.1016/j.ab.2024.115721
Publication status	Published - Feb 2025

Keywords

3D printer
Gel electrophoresis
Liquid handler
Optical sensor

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10.1016/j.ab.2024.115721Licence: CC BY

Cite this

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abstract = "In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.",

keywords = "3D printer, Gel electrophoresis, Liquid handler, Optical sensor",

author = "Tao Tao and Alexey Kopyl and Yuki Yew and Fawaz El-Dani and Abid, {Hassan Ali} and Thomas Hiscox and Liew, {Oi Wah} and Ng, {Tuck Wah}",

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doi = "10.1016/j.ab.2024.115721",

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T1 - Sample loading in gel electrophoresis using adapted 3D printers

AU - Tao, Tao

AU - Kopyl, Alexey

AU - Yew, Yuki

AU - El-Dani, Fawaz

AU - Abid, Hassan Ali

AU - Hiscox, Thomas

AU - Liew, Oi Wah

AU - Ng, Tuck Wah

PY - 2025/2

Y1 - 2025/2

N2 - In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.

AB - In gel electrophoresis, samples that are dispensed too high above or too low into the wells result in sub-optimal outcomes. Here, an adapted 3D printer liquid handler equipped with an optical sensor was found to attain vertical sample delivery positionings at a standard deviation over mean ratio of 0.008. This illustrated high accuracy and repeatability outcomes achieved using automation that is cost effective and low in technical knowledge demand.

KW - 3D printer

KW - Gel electrophoresis

KW - Liquid handler

KW - Optical sensor

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DO - 10.1016/j.ab.2024.115721

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VL - 697

JO - Analytical Biochemistry

JF - Analytical Biochemistry

M1 - 115721

ER -

Sample loading in gel electrophoresis using adapted 3D printers

Abstract

Keywords

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