Salmonella typhimurium Infections in Mice Deficient in Interleukin-4 Production: Role of IL-4 in Infection-Associated Pathology

Paul Everest, Jenny Allen, Anastasia Papakonstantinopoulou, Pietro Mastroeni, Mark Roberts, Gordon Dougan

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Mice harboring mutations in the IL-4 gene (IL-4-/-) were infected with a range of Salmonella typhimurium HWSH derivatives using different routes of infection. Compared with IL-4+/+ mice, IL-4-/- mice exhibited a delayed time to death following infection with wild-type S. typhimurium HWSH. Croups of IL-4+/+ mice infected with S. typhimurium HWSH purE, a less virulent derivative, showed sporadic deaths and harbored micro- or macroabscesses in their tissues, particularly associated with the liver. However, IL-4-/- mice infected with similar doses of S. typhimurium HWSH purE bacteria were resistant to killing and failed to develop detectable abscesses. Abscess formation in IL-4-/- mice could be induced by i.v. administration of rlL-4 during the S. typhimurium HWSH purE infection. The immune response in both IL-4-/- and IL-4+/+ mice was of the Th1-type. Viable salmonella bacteria could be found associated with abscesses. Both IL-4-/- and IL-4+/+ mice were resistant to killing by S. typhimurium aroA.

Original languageEnglish
Pages (from-to)1820-1827
Number of pages8
JournalJournal of Immunology
Issue number4
Publication statusPublished - 15 Aug 1997
Externally publishedYes

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