Salmonella typhimurium ΔaroA ΔaroD mutants expressing a foreign recombinant protein induce specific major histocompatibility complex class I- restricted cytotoxic T lymphocytes in mice

S. J. Turner, F. R. Carbone, R. A. Strugnell

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Abstract

Recombinant Salmonella typhimurium aroA aroD mutants which expressed ovalbumin were constructed. The two expression constructs used were based on either pUC18 or pBR322. The pBR322-based construct was more stable in vitro and in vivo than the pUC-based construct. Salmonellae containing the stable pBR322-based plasmid induced major histocompatibility complex (MHC) class-I- restricted cytotoxic T lymphocytes (CTL), in contrast to salmonellae containing the pUC18-based expression construct. The priming of MHC class I- restricted CTL was increased by multiple immunizations. The study described in this report suggests that S. typhimurium Δaro mutants have the capacity to induce MHC class I-restricted CTL against carried antigens and that MHC class I-restricted CTL responses require stable in vivo expression of the target antigen. Further, the results indicate that the Salmonella typhi Δaro mutants currently undergoing evaluation in studies with humans may be good carriers of viral antigens with CTL determinants.

Original languageEnglish
Pages (from-to)5374-5380
Number of pages7
JournalInfection and Immunity
Volume61
Issue number12
Publication statusPublished - 1 Jan 1993
Externally publishedYes

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