Salmonella

Immune responses and vaccines

Pietro Mastroeni, J. A. Chabalgoity, S. J. Dunstan, D. J. Maskell, G. Dougan

Research output: Contribution to journalReview ArticleResearchpeer-review

Abstract

Salmonella infections are a serious medical and veterinary problem world-wide and cause concern in the food industry. Vaccination is an effective tool for the prevention of Salmonella infections. Host resistance to Salmonella relies initially on the production of inflammatory cytokines leading to the infiltration of activated inflammatory cells in the tissues. Thereafter, T- and B-cell dependent specific immunity develops allowing the clearance of Salmonella microorganisms from the tissues and the establishment of long-lasting acquired immunity to re-infection. The increased resistance that develops after primary infection/ vaccination requires T-cells, cytokines such as IFNγ, TNFα and IL12 in addition to opsonising antibody. However, for reasons that are not fully understood, seroconversion and/or the presence of detectable T-cell memory do not always correlate with the development of acquired resistance to infection. Whole-cell killed vaccines and subunit vaccines are used in the prevention of Salmonella infection in animals and in humans with variable results. A number of early live Salmonella vaccines derived empirically by chemical or u.v. mutagenesis proved to be immunogenic and protective and are still in use despite the need for repeated parenteral administration. Recent progress in the knowledge of the genetics of Salmonella virulence and modern recombinant DNA technology offers the possibility to introduce multiple, defined, attenuating and irreversible mutations into the bacterial genome. This has recently allowed the development of Salmonella strains devoid of significant side effects, but still capable of inducing solid immunity after single oral administration. Live attenuated Salmonella vaccines have been used for the expression of heterologous antigens/proteins that can be successfully delivered to the immune system. Furthermore, Salmonella can transfer plasmids encoding foreign antigens under the control of eukaryotic promoters (DNA vaccines) to antigen-presenting preseiiting cells resulting in targeted delivery of DNA vaccines to these cells. Despite the great recent advances in the development of Salmonella vaccines, a large proportion of the work has been conducted in laboratory rodents and more research in other animal species is required.

Original languageEnglish
Pages (from-to)132-164
Number of pages33
JournalVeterinary Journal
Volume161
Issue number2
DOIs
Publication statusPublished - 1 Jan 2001
Externally publishedYes

Keywords

  • Immunity
  • Infection
  • Salmonella
  • Vaccines
  • Veterinary

Cite this

Mastroeni, P., Chabalgoity, J. A., Dunstan, S. J., Maskell, D. J., & Dougan, G. (2001). Salmonella: Immune responses and vaccines. Veterinary Journal, 161(2), 132-164. https://doi.org/10.1053/tvjl.2000.0502
Mastroeni, Pietro ; Chabalgoity, J. A. ; Dunstan, S. J. ; Maskell, D. J. ; Dougan, G. / Salmonella : Immune responses and vaccines. In: Veterinary Journal. 2001 ; Vol. 161, No. 2. pp. 132-164.
@article{685d0414487644ad8c4d968f4a480ba4,
title = "Salmonella: Immune responses and vaccines",
abstract = "Salmonella infections are a serious medical and veterinary problem world-wide and cause concern in the food industry. Vaccination is an effective tool for the prevention of Salmonella infections. Host resistance to Salmonella relies initially on the production of inflammatory cytokines leading to the infiltration of activated inflammatory cells in the tissues. Thereafter, T- and B-cell dependent specific immunity develops allowing the clearance of Salmonella microorganisms from the tissues and the establishment of long-lasting acquired immunity to re-infection. The increased resistance that develops after primary infection/ vaccination requires T-cells, cytokines such as IFNγ, TNFα and IL12 in addition to opsonising antibody. However, for reasons that are not fully understood, seroconversion and/or the presence of detectable T-cell memory do not always correlate with the development of acquired resistance to infection. Whole-cell killed vaccines and subunit vaccines are used in the prevention of Salmonella infection in animals and in humans with variable results. A number of early live Salmonella vaccines derived empirically by chemical or u.v. mutagenesis proved to be immunogenic and protective and are still in use despite the need for repeated parenteral administration. Recent progress in the knowledge of the genetics of Salmonella virulence and modern recombinant DNA technology offers the possibility to introduce multiple, defined, attenuating and irreversible mutations into the bacterial genome. This has recently allowed the development of Salmonella strains devoid of significant side effects, but still capable of inducing solid immunity after single oral administration. Live attenuated Salmonella vaccines have been used for the expression of heterologous antigens/proteins that can be successfully delivered to the immune system. Furthermore, Salmonella can transfer plasmids encoding foreign antigens under the control of eukaryotic promoters (DNA vaccines) to antigen-presenting preseiiting cells resulting in targeted delivery of DNA vaccines to these cells. Despite the great recent advances in the development of Salmonella vaccines, a large proportion of the work has been conducted in laboratory rodents and more research in other animal species is required.",
keywords = "Immunity, Infection, Salmonella, Vaccines, Veterinary",
author = "Pietro Mastroeni and Chabalgoity, {J. A.} and Dunstan, {S. J.} and Maskell, {D. J.} and G. Dougan",
year = "2001",
month = "1",
day = "1",
doi = "10.1053/tvjl.2000.0502",
language = "English",
volume = "161",
pages = "132--164",
journal = "Veterinary Journal",
issn = "1090-0233",
publisher = "Elsevier",
number = "2",

}

Mastroeni, P, Chabalgoity, JA, Dunstan, SJ, Maskell, DJ & Dougan, G 2001, 'Salmonella: Immune responses and vaccines', Veterinary Journal, vol. 161, no. 2, pp. 132-164. https://doi.org/10.1053/tvjl.2000.0502

Salmonella : Immune responses and vaccines. / Mastroeni, Pietro; Chabalgoity, J. A.; Dunstan, S. J.; Maskell, D. J.; Dougan, G.

In: Veterinary Journal, Vol. 161, No. 2, 01.01.2001, p. 132-164.

Research output: Contribution to journalReview ArticleResearchpeer-review

TY - JOUR

T1 - Salmonella

T2 - Immune responses and vaccines

AU - Mastroeni, Pietro

AU - Chabalgoity, J. A.

AU - Dunstan, S. J.

AU - Maskell, D. J.

AU - Dougan, G.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Salmonella infections are a serious medical and veterinary problem world-wide and cause concern in the food industry. Vaccination is an effective tool for the prevention of Salmonella infections. Host resistance to Salmonella relies initially on the production of inflammatory cytokines leading to the infiltration of activated inflammatory cells in the tissues. Thereafter, T- and B-cell dependent specific immunity develops allowing the clearance of Salmonella microorganisms from the tissues and the establishment of long-lasting acquired immunity to re-infection. The increased resistance that develops after primary infection/ vaccination requires T-cells, cytokines such as IFNγ, TNFα and IL12 in addition to opsonising antibody. However, for reasons that are not fully understood, seroconversion and/or the presence of detectable T-cell memory do not always correlate with the development of acquired resistance to infection. Whole-cell killed vaccines and subunit vaccines are used in the prevention of Salmonella infection in animals and in humans with variable results. A number of early live Salmonella vaccines derived empirically by chemical or u.v. mutagenesis proved to be immunogenic and protective and are still in use despite the need for repeated parenteral administration. Recent progress in the knowledge of the genetics of Salmonella virulence and modern recombinant DNA technology offers the possibility to introduce multiple, defined, attenuating and irreversible mutations into the bacterial genome. This has recently allowed the development of Salmonella strains devoid of significant side effects, but still capable of inducing solid immunity after single oral administration. Live attenuated Salmonella vaccines have been used for the expression of heterologous antigens/proteins that can be successfully delivered to the immune system. Furthermore, Salmonella can transfer plasmids encoding foreign antigens under the control of eukaryotic promoters (DNA vaccines) to antigen-presenting preseiiting cells resulting in targeted delivery of DNA vaccines to these cells. Despite the great recent advances in the development of Salmonella vaccines, a large proportion of the work has been conducted in laboratory rodents and more research in other animal species is required.

AB - Salmonella infections are a serious medical and veterinary problem world-wide and cause concern in the food industry. Vaccination is an effective tool for the prevention of Salmonella infections. Host resistance to Salmonella relies initially on the production of inflammatory cytokines leading to the infiltration of activated inflammatory cells in the tissues. Thereafter, T- and B-cell dependent specific immunity develops allowing the clearance of Salmonella microorganisms from the tissues and the establishment of long-lasting acquired immunity to re-infection. The increased resistance that develops after primary infection/ vaccination requires T-cells, cytokines such as IFNγ, TNFα and IL12 in addition to opsonising antibody. However, for reasons that are not fully understood, seroconversion and/or the presence of detectable T-cell memory do not always correlate with the development of acquired resistance to infection. Whole-cell killed vaccines and subunit vaccines are used in the prevention of Salmonella infection in animals and in humans with variable results. A number of early live Salmonella vaccines derived empirically by chemical or u.v. mutagenesis proved to be immunogenic and protective and are still in use despite the need for repeated parenteral administration. Recent progress in the knowledge of the genetics of Salmonella virulence and modern recombinant DNA technology offers the possibility to introduce multiple, defined, attenuating and irreversible mutations into the bacterial genome. This has recently allowed the development of Salmonella strains devoid of significant side effects, but still capable of inducing solid immunity after single oral administration. Live attenuated Salmonella vaccines have been used for the expression of heterologous antigens/proteins that can be successfully delivered to the immune system. Furthermore, Salmonella can transfer plasmids encoding foreign antigens under the control of eukaryotic promoters (DNA vaccines) to antigen-presenting preseiiting cells resulting in targeted delivery of DNA vaccines to these cells. Despite the great recent advances in the development of Salmonella vaccines, a large proportion of the work has been conducted in laboratory rodents and more research in other animal species is required.

KW - Immunity

KW - Infection

KW - Salmonella

KW - Vaccines

KW - Veterinary

UR - http://www.scopus.com/inward/record.url?scp=0034965277&partnerID=8YFLogxK

U2 - 10.1053/tvjl.2000.0502

DO - 10.1053/tvjl.2000.0502

M3 - Review Article

VL - 161

SP - 132

EP - 164

JO - Veterinary Journal

JF - Veterinary Journal

SN - 1090-0233

IS - 2

ER -

Mastroeni P, Chabalgoity JA, Dunstan SJ, Maskell DJ, Dougan G. Salmonella: Immune responses and vaccines. Veterinary Journal. 2001 Jan 1;161(2):132-164. https://doi.org/10.1053/tvjl.2000.0502