Salmonella enterica serovar Typhimurium interaction with dendritic cells: Impact of the sifA gene

Liljana Petrovska, Richard J. Aspinall, Li Barber, Simon Clare, Cameron P. Simmons, Richard Stratford, Shahid A. Khan, Nicholas R. Lemoine, Gad Frankel, David W. Holden, Gordon Dougan

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)

Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) and several mutant derivatives were able to enter efficiently murine bone marrow-derived dendritic cells using mechanisms predominantly independent of the Salmonella pathogenicity island 1 type III secretion system. The levels of intracellular bacteria did not increase significantly over many hours after invasion. Using fluid endocytic tracers and other markers, S. Typhimurium-containing vacuoles (SCVs) were physically distinguishable from early endocytic compartments. Fifty to eighty per cent of SCVs harbouring wild-type S. Typhimurium or aroA, invH and ssaV mutant derivatives were associated with late endosome markers. In contrast, S. Typhimurium sifA was shown to escape the SCVs into the cytosol of infected dendritic cells. S. Typhimurium aroC sifA was more efficient than S. Typhimurium aroC in delivering a eukaryotic promoter-driven green fluorescent protein reporter gene for expression in dendritic cells. In contrast, S. Typhimurium aroC sifA did not detectably increase the efficiency of MHC class I presentation of the model antigen ovalbumin to T cells compared to a similar aroC derivative. Mice infected with the S. Typhimurium aroC sifA expressing ovalbumin did not develop detectabiy enhanced levels of cytotoxic T cell or interferon-γ production compared to S. Typhimurium aroC derivatives.

Original languageEnglish
Pages (from-to)1071-1084
Number of pages14
JournalCellular Microbiology
Volume6
Issue number11
DOIs
Publication statusPublished - 1 Nov 2004
Externally publishedYes

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