Saccharomyces cerevisiae expression vectors with thrombin-cleavable N- and C-terminal 6x(His) tags

Onisha Patel, Ross Fernley, Ian Macreadie

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9 Citations (Scopus)

Abstract

General-purpose yeast expression vectors for convenient cloning and production of proteins with N- or C-terminal His6 tags that can be efficiently removed with thrombin have been developed. To the parental yeast-E. coli shuttle vectors that have convenient copper-inducible expression, two selectable markers and LEU2d vector amplification, this development adds substantial versatility to product recovery.
Original languageEnglish
Pages (from-to)331 - 334
Number of pages4
JournalBiotechnology Letters
Volume25
Issue number4
Publication statusPublished - 2003

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