TY - JOUR
T1 - s8ORF2 protein of infectious salmon anaemia virus is a RNA-silencing suppressor and interacts with Salmon salar Mov10 (SsMov10) of the host RNAi machinery
AU - Thukral, Vandana
AU - Varshney, Bhavna
AU - Ramly, Rimatulhana B.
AU - Ponia, Sanket S.
AU - Mishra, Sumona Karjee
AU - Olsen, Christel M.
AU - Banerjea, Akhil C.
AU - Mukherjee, Sunil K.
AU - Zaidi, Rana
AU - Rimstad, Espen
AU - Lal, Sunil K.
N1 - Funding Information:
Acknowledgements This research was funded by the Department of Biotechnology Indo-Norwegian grant, Govt. of India; the Indian Council for Medical Research fellowship for VT, research grant 183196/ S40 from the Research Council of Norway, and internal funds from the School of Science, Monash University Malaysia. The authors are grateful for technical help from Stine Braaen.
Publisher Copyright:
© 2017, Springer Science+Business Media, LLC, part of Springer Nature.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/4
Y1 - 2018/4
N2 - The infectious salmon anaemia virus (ISAV) is a piscine virus, a member of Orthomyxoviridae family. It encodes at least 10 proteins from eight negative-strand RNA segments. Since ISAV belongs to the same virus family as Influenza A virus, with similarities in protein functions, they may hence be characterised by analogy. Like NS1 protein of Influenza A virus, s8ORF2 of ISAV is implicated in interferon antagonism and RNA-binding functions. In this study, we investigated the role of s8ORF2 in RNAi suppression in a well-established Agrobacterium transient suppression assay in stably silenced transgenic Nicotiana xanthi. In addition, s8ORF2 was identified as a novel interactor with SsMov10, a key molecule responsible for RISC assembly and maturation in the RNAi pathway. This study thus sheds light on a novel route undertaken by viral proteins in promoting viral growth, using the host RNAi machinery.
AB - The infectious salmon anaemia virus (ISAV) is a piscine virus, a member of Orthomyxoviridae family. It encodes at least 10 proteins from eight negative-strand RNA segments. Since ISAV belongs to the same virus family as Influenza A virus, with similarities in protein functions, they may hence be characterised by analogy. Like NS1 protein of Influenza A virus, s8ORF2 of ISAV is implicated in interferon antagonism and RNA-binding functions. In this study, we investigated the role of s8ORF2 in RNAi suppression in a well-established Agrobacterium transient suppression assay in stably silenced transgenic Nicotiana xanthi. In addition, s8ORF2 was identified as a novel interactor with SsMov10, a key molecule responsible for RISC assembly and maturation in the RNAi pathway. This study thus sheds light on a novel route undertaken by viral proteins in promoting viral growth, using the host RNAi machinery.
KW - Infectious Salmon Anaemia Virus
KW - Salmon salar Mov10
KW - Segment 8 Open Reading Frame 2
KW - SsMov10
KW - Viral suppressor of RNA silencing
UR - http://www.scopus.com/inward/record.url?scp=85037349577&partnerID=8YFLogxK
U2 - 10.1007/s11262-017-1526-z
DO - 10.1007/s11262-017-1526-z
M3 - Article
C2 - 29218433
AN - SCOPUS:85037349577
SN - 0920-8569
VL - 54
SP - 199
EP - 214
JO - Virus Genes
JF - Virus Genes
IS - 2
ER -