s8ORF2 protein of infectious salmon anaemia virus is a RNA-silencing suppressor and interacts with Salmon salar Mov10 (SsMov10) of the host RNAi machinery

Vandana Thukral, Bhavna Varshney, Rimatulhana B. Ramly, Sanket S. Ponia, Sumona Karjee Mishra, Christel M. Olsen, Akhil C. Banerjea, Sunil K. Mukherjee, Rana Zaidi, Espen Rimstad, Sunil K. Lal

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6 Citations (Scopus)


The infectious salmon anaemia virus (ISAV) is a piscine virus, a member of Orthomyxoviridae family. It encodes at least 10 proteins from eight negative-strand RNA segments. Since ISAV belongs to the same virus family as Influenza A virus, with similarities in protein functions, they may hence be characterised by analogy. Like NS1 protein of Influenza A virus, s8ORF2 of ISAV is implicated in interferon antagonism and RNA-binding functions. In this study, we investigated the role of s8ORF2 in RNAi suppression in a well-established Agrobacterium transient suppression assay in stably silenced transgenic Nicotiana xanthi. In addition, s8ORF2 was identified as a novel interactor with SsMov10, a key molecule responsible for RISC assembly and maturation in the RNAi pathway. This study thus sheds light on a novel route undertaken by viral proteins in promoting viral growth, using the host RNAi machinery.

Original languageEnglish
Pages (from-to)199-214
Number of pages16
JournalVirus Genes
Issue number2
Publication statusPublished - Apr 2018


  • Infectious Salmon Anaemia Virus
  • Salmon salar Mov10
  • Segment 8 Open Reading Frame 2
  • SsMov10
  • Viral suppressor of RNA silencing

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