Royal Society of Tropical Medicine and Hygiene Meeting at Manson House, London, 14 December 1995. Enteropathogenic Escherichia coli - mucosal infection models

Gad Frankel, Alan D. Phillips, Susan Hicks, Gordon Dougan

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The formation of attaching and effacing (A/E) lesions is central to the pathogenesis of enteropathogenic Escherichia coli (EPEC)-mediated disease in humans and Citrobacter rodentium-mediated transmissible colonic hyperplasia in mice. Closely related outer membrane proteins, known as intimins, are required for formation of the A/E lesion by both EPEC and C. rodentium. In this study we found similar ultrastructural damage in small intestinal biopsies from an EPEC-infected child and large bowel specimens from C. rodentium-infected mice. The C. rodentium-infected large bowel biopsies revealed massive hyperplastic reactions and the infected human small intestinal biopsies showed an increase in total crypt cell number and mitotic index. EPEC-infected small intestinal organ cultures revealed bacteria adhering in a localized pattern and evidence of A/E lesions. Covaspheres® coated with a biologically active cell-binding domain of intimin also adhered to cells in a localized fashion but did not induce the characteristic A/E lesions.

Original languageEnglish
Pages (from-to)347-352
Number of pages6
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Issue number4
Publication statusPublished - 1 Jan 1996
Externally publishedYes


  • Citrobacter rodentium
  • Enteropathogenic Escherichia coli
  • Intestinal lesions
  • Light and electron microscopy
  • Pathogenesis

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