Rotenone and MPP+ preferentially redistribute apoptosis-inducing factor in apoptotic dopamine neurons

Maria Lourdes Regina Lim, Linda Mercer, Phillip Nagley, Philip M Beart

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17 Citations (Scopus)


Rotenone and 1-methyl-4-phenylpyridinium produce parkinsonian models and we determined whether their mitochondrially mediated actions differentially redistributed the apoptogenic proteins, apoptosis-inducing factor and cytochrome c. Cultured rat mesencephalic dopamine neurons were exposed to rotenone (30 nM) and 1-methyl-4-phenylpyridinium (300 muM, 24 and 48 h) and apoptosis and mitochondrial redistribution of cytochrome c or apoptosis-inducing factor were quantified. Tyrosine hydroxylase-positive dopamine neurons underwent apoptosis (shrinkage, less neurites) and 40 released apoptosis-inducing factor with rotenone (24 h), whereas cytochrome c release reached this value at 48 h when 70 of cells had released apoptosis-inducing factor-positive. 1-Methyl-4-phenylpyridinium produced similar redistribution patterns for both proteins. Preferential redistribution of apoptosis-inducing factor before cytochrome c in dopamine neurons indicates caspase-independent mitochondrial proapoptotic signalling predominates in these parkinsonian models.
Original languageEnglish
Pages (from-to)307 - 312
Number of pages6
Issue number4
Publication statusPublished - 2007

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