TY - JOUR
T1 - Rosmarinic acid-rich ethanolic extract of Orthosiphon stamineus ameliorates cognitive and hippocampal long-term potentiation in chronic cerebral hypoperfusion rat model
AU - Hassan, Zurina
AU - Kumaran, Kesevan Rajah
AU - Chear, Nelson Jeng Yeou
AU - Bakar, Siti Najmi Syuhadaa
AU - Retinasamy, Thaarvena
AU - Bhuvanendran, Saatheeyavaane
AU - Majeed, Amin Malik Abdul
AU - Shaikh, Mohd Farooq
AU - Othman, Iekhsan
N1 - Funding Information:
The project is supported by Universiti Sains Malaysia (Research University Individual) grant (1001/CDADAH/8012302) and NKEA Research Grant Scheme (NRGS), Ministry of Agriculture and Agro-Based Industry Malaysia (Grant No. NH1014D066). NJYC would like to acknowledge Universiti Sains Malaysia for awarding the USM Fellowship Scheme.
Publisher Copyright:
© 2023 by Hassan et al.
PY - 2023/8/27
Y1 - 2023/8/27
N2 - Chronic cerebral hypoperfusion (CCH) is one of the main causes of vascular dementia caused by the reduced blood flow to the brain. Orthosiphon stamineus (OS) is a medicinal herb exhibiting pronounced neuroprotective, anti-oxidant and anti-inflammatory activities due to its high rosmarinic acid content. This study investigated the nootropic effect of OS ethanolic extract on cognitive functions in CCH rats. CCH was developed by permanent bilateral occlusion of the common carotid artery (PBOCCA). Passive avoidance task (PAT) and Morris water maze (MWM) test were conducted to evaluate cognitive functions followed by in vivo long-term potentiation (LTP) for assessing neuroplasticity. The rosmarinic acid content of OS ethanolic extract was quantified using a validated HPLC-PDA. Treatment with OS ethanolic extract significantly increased step-through latency in the PAT, decreased escape latency at a low dose of OS extract in the MWM and rescued the LTP impairment at the highest dose in CCH rats. These results strongly support the effectiveness of rosmarinic acid-rich OS extract (5.088 % w/w) in treating pathological vascular dementia caused by CCH.
AB - Chronic cerebral hypoperfusion (CCH) is one of the main causes of vascular dementia caused by the reduced blood flow to the brain. Orthosiphon stamineus (OS) is a medicinal herb exhibiting pronounced neuroprotective, anti-oxidant and anti-inflammatory activities due to its high rosmarinic acid content. This study investigated the nootropic effect of OS ethanolic extract on cognitive functions in CCH rats. CCH was developed by permanent bilateral occlusion of the common carotid artery (PBOCCA). Passive avoidance task (PAT) and Morris water maze (MWM) test were conducted to evaluate cognitive functions followed by in vivo long-term potentiation (LTP) for assessing neuroplasticity. The rosmarinic acid content of OS ethanolic extract was quantified using a validated HPLC-PDA. Treatment with OS ethanolic extract significantly increased step-through latency in the PAT, decreased escape latency at a low dose of OS extract in the MWM and rescued the LTP impairment at the highest dose in CCH rats. These results strongly support the effectiveness of rosmarinic acid-rich OS extract (5.088 % w/w) in treating pathological vascular dementia caused by CCH.
KW - chronic cerebral hypoperfused rats
KW - cognitive function
KW - Morris-water-maze
KW - O. stamineus
KW - passive avoidance task
UR - http://www.scopus.com/inward/record.url?scp=85173997654&partnerID=8YFLogxK
U2 - 10.31117/neuroscirn.v6i3.196
DO - 10.31117/neuroscirn.v6i3.196
M3 - Article
AN - SCOPUS:85173997654
SN - 2576-828X
VL - 6
JO - Neuroscience Research Notes
JF - Neuroscience Research Notes
IS - 3
M1 - 196
ER -