Roles of nuclear trafficking in infection by cytoplasmic negative-strand RNA viruses: Paramyxoviruses and beyond

Research output: Contribution to journalReview ArticleOtherpeer-review

Abstract

Genome replication and virion production by most negative-sense RNA viruses (NSVs) occurs exclusively in the cytoplasm, but many NSV-expressed proteins undergo active nucleocytoplasmic trafficking via signals that exploit cellular nuclear transport pathways. Nuclear trafficking has been reported both for NSV accessory proteins (including isoforms of the rabies virus phosphoprotein, and V,W and C proteins of paramyxoviruses) and for structural proteins. Trafficking of the former is thought to enable accessory functions in viral modulation of antiviral responses including the type I IFN system, but the intranuclear roles of structural proteins such as nucleocapsid and matrix proteins, which have critical roles in extranuclear replication and viral assembly, are less clear. Nevertheless, nuclear trafficking of matrix protein has been reported to be critical for efficient production of Nipah virus and Respiratory syncytial virus, and nuclear localization of nucleocapsid protein of several morbilliviruses has been linked to mechanisms of immune evasion. Together, these data point to the nucleus as a significant host interface for viral proteins during infection by NSVs with otherwise cytoplasmic life cycles. Importantly, several lines of evidence now suggest that nuclear trafficking of these proteins may be critical to pathogenesis and thus could provide new targets for vaccine development and antiviral therapies.

Original languageEnglish
Article number000575
Pages (from-to)2463-2481
Number of pages19
JournalJournal of General Virology
Volume97
Issue number10
DOIs
Publication statusPublished - 13 Oct 2016

Keywords

  • Importin
  • Lyssavirus
  • Negative strand RNA virus
  • Nuclear import
  • Nucleus
  • Paramyxovirus

Cite this

@article{20f16494246143c99be27d7324a2102c,
title = "Roles of nuclear trafficking in infection by cytoplasmic negative-strand RNA viruses: Paramyxoviruses and beyond",
abstract = "Genome replication and virion production by most negative-sense RNA viruses (NSVs) occurs exclusively in the cytoplasm, but many NSV-expressed proteins undergo active nucleocytoplasmic trafficking via signals that exploit cellular nuclear transport pathways. Nuclear trafficking has been reported both for NSV accessory proteins (including isoforms of the rabies virus phosphoprotein, and V,W and C proteins of paramyxoviruses) and for structural proteins. Trafficking of the former is thought to enable accessory functions in viral modulation of antiviral responses including the type I IFN system, but the intranuclear roles of structural proteins such as nucleocapsid and matrix proteins, which have critical roles in extranuclear replication and viral assembly, are less clear. Nevertheless, nuclear trafficking of matrix protein has been reported to be critical for efficient production of Nipah virus and Respiratory syncytial virus, and nuclear localization of nucleocapsid protein of several morbilliviruses has been linked to mechanisms of immune evasion. Together, these data point to the nucleus as a significant host interface for viral proteins during infection by NSVs with otherwise cytoplasmic life cycles. Importantly, several lines of evidence now suggest that nuclear trafficking of these proteins may be critical to pathogenesis and thus could provide new targets for vaccine development and antiviral therapies.",
keywords = "Importin, Lyssavirus, Negative strand RNA virus, Nuclear import, Nucleus, Paramyxovirus",
author = "Audsley, {Michelle D.} and Jans, {David A.} and Moseley, {Gregory W.}",
year = "2016",
month = "10",
day = "13",
doi = "10.1099/jgv.0.000575",
language = "English",
volume = "97",
pages = "2463--2481",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Microbiology Society",
number = "10",

}

Roles of nuclear trafficking in infection by cytoplasmic negative-strand RNA viruses : Paramyxoviruses and beyond. / Audsley, Michelle D.; Jans, David A.; Moseley, Gregory W.

In: Journal of General Virology, Vol. 97, No. 10, 000575, 13.10.2016, p. 2463-2481.

Research output: Contribution to journalReview ArticleOtherpeer-review

TY - JOUR

T1 - Roles of nuclear trafficking in infection by cytoplasmic negative-strand RNA viruses

T2 - Paramyxoviruses and beyond

AU - Audsley, Michelle D.

AU - Jans, David A.

AU - Moseley, Gregory W.

PY - 2016/10/13

Y1 - 2016/10/13

N2 - Genome replication and virion production by most negative-sense RNA viruses (NSVs) occurs exclusively in the cytoplasm, but many NSV-expressed proteins undergo active nucleocytoplasmic trafficking via signals that exploit cellular nuclear transport pathways. Nuclear trafficking has been reported both for NSV accessory proteins (including isoforms of the rabies virus phosphoprotein, and V,W and C proteins of paramyxoviruses) and for structural proteins. Trafficking of the former is thought to enable accessory functions in viral modulation of antiviral responses including the type I IFN system, but the intranuclear roles of structural proteins such as nucleocapsid and matrix proteins, which have critical roles in extranuclear replication and viral assembly, are less clear. Nevertheless, nuclear trafficking of matrix protein has been reported to be critical for efficient production of Nipah virus and Respiratory syncytial virus, and nuclear localization of nucleocapsid protein of several morbilliviruses has been linked to mechanisms of immune evasion. Together, these data point to the nucleus as a significant host interface for viral proteins during infection by NSVs with otherwise cytoplasmic life cycles. Importantly, several lines of evidence now suggest that nuclear trafficking of these proteins may be critical to pathogenesis and thus could provide new targets for vaccine development and antiviral therapies.

AB - Genome replication and virion production by most negative-sense RNA viruses (NSVs) occurs exclusively in the cytoplasm, but many NSV-expressed proteins undergo active nucleocytoplasmic trafficking via signals that exploit cellular nuclear transport pathways. Nuclear trafficking has been reported both for NSV accessory proteins (including isoforms of the rabies virus phosphoprotein, and V,W and C proteins of paramyxoviruses) and for structural proteins. Trafficking of the former is thought to enable accessory functions in viral modulation of antiviral responses including the type I IFN system, but the intranuclear roles of structural proteins such as nucleocapsid and matrix proteins, which have critical roles in extranuclear replication and viral assembly, are less clear. Nevertheless, nuclear trafficking of matrix protein has been reported to be critical for efficient production of Nipah virus and Respiratory syncytial virus, and nuclear localization of nucleocapsid protein of several morbilliviruses has been linked to mechanisms of immune evasion. Together, these data point to the nucleus as a significant host interface for viral proteins during infection by NSVs with otherwise cytoplasmic life cycles. Importantly, several lines of evidence now suggest that nuclear trafficking of these proteins may be critical to pathogenesis and thus could provide new targets for vaccine development and antiviral therapies.

KW - Importin

KW - Lyssavirus

KW - Negative strand RNA virus

KW - Nuclear import

KW - Nucleus

KW - Paramyxovirus

UR - http://www.scopus.com/inward/record.url?scp=84991736601&partnerID=8YFLogxK

U2 - 10.1099/jgv.0.000575

DO - 10.1099/jgv.0.000575

M3 - Review Article

VL - 97

SP - 2463

EP - 2481

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 10

M1 - 000575

ER -