Role of organic carbon, nitrate and ferrous iron on the partitioning between denitrification and DNRA in constructed stormwater urban wetlands

Md Moklesur Rahman, Keryn L. Roberts, Michael R. Grace, Adam J. Kessler, Perran L.M. Cook

Research output: Contribution to journalArticleResearchpeer-review

3 Citations (Scopus)

Abstract

Denitrification (DNF) and dissimilatory nitrate reduction to ammonium (DNRA) are two competing nitrate reduction pathways that remove or recycle nitrogen, respectively. However, factors controlling the partitioning between these two pathways are manifold and our understanding of these factors is critical for the management of N loads in constructed wetlands. An important factor that controls DNRA in an aquatic ecosystem is the electron donor, commonly organic carbon (OC) or alternatively ferrous iron and sulfide. In this study, we investigated the role of natural organic carbon (NOC) and acetate at different OC/NO 3 ratios and ferrous iron on the partitioning between DNF and DNRA using the 15 N-tracer method in slurries from four constructed stormwater urban wetlands in Melbourne, Australia. The carbon and nitrate experiments revealed that DNF dominated at all OC/NO 3 ratios. The higher DNF and DNRA rates observed after the addition of NOC indicates that nitrate reduction was enhanced more by NOC than acetate. Moreover, addition of NOC in slurries stimulated DNRA more than DNF. Interestingly, slurries amended with Fe 2+ showed that Fe 2+ had significant control on the balance between DNF and DNRA. From two out of four wetlands, a significant increase in DNRA rates (p < .05) at the cost of DNF in the presence of available Fe 2+ suggests DNRA is coupled to Fe 2+ oxidation. Rates of DNRA increased 1.5–3.5 times in the Fe 2+ treatment compared to the control. Overall, our study provides direct evidence that DNRA is linked to Fe 2+ oxidation in some wetland sediments and highlights the role of Fe 2+ in controlling the partitioning between removal (DNF) and recycling (DNRA) of bioavailable N in stormwater urban constructed wetlands. In our study we also measured anammox and found that it was always <0.05% of total nitrate reduction in these sediments.

Original languageEnglish
Pages (from-to)608-617
Number of pages10
JournalScience of the Total Environment
Volume666
DOIs
Publication statusPublished - 20 May 2019

Keywords

  • Denitrification
  • DNRA
  • Ferrous Fe
  • Organic carbon
  • Wetland

Cite this

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title = "Role of organic carbon, nitrate and ferrous iron on the partitioning between denitrification and DNRA in constructed stormwater urban wetlands",
abstract = "Denitrification (DNF) and dissimilatory nitrate reduction to ammonium (DNRA) are two competing nitrate reduction pathways that remove or recycle nitrogen, respectively. However, factors controlling the partitioning between these two pathways are manifold and our understanding of these factors is critical for the management of N loads in constructed wetlands. An important factor that controls DNRA in an aquatic ecosystem is the electron donor, commonly organic carbon (OC) or alternatively ferrous iron and sulfide. In this study, we investigated the role of natural organic carbon (NOC) and acetate at different OC/NO 3 − ratios and ferrous iron on the partitioning between DNF and DNRA using the 15 N-tracer method in slurries from four constructed stormwater urban wetlands in Melbourne, Australia. The carbon and nitrate experiments revealed that DNF dominated at all OC/NO 3 − ratios. The higher DNF and DNRA rates observed after the addition of NOC indicates that nitrate reduction was enhanced more by NOC than acetate. Moreover, addition of NOC in slurries stimulated DNRA more than DNF. Interestingly, slurries amended with Fe 2+ showed that Fe 2+ had significant control on the balance between DNF and DNRA. From two out of four wetlands, a significant increase in DNRA rates (p < .05) at the cost of DNF in the presence of available Fe 2+ suggests DNRA is coupled to Fe 2+ oxidation. Rates of DNRA increased 1.5–3.5 times in the Fe 2+ treatment compared to the control. Overall, our study provides direct evidence that DNRA is linked to Fe 2+ oxidation in some wetland sediments and highlights the role of Fe 2+ in controlling the partitioning between removal (DNF) and recycling (DNRA) of bioavailable N in stormwater urban constructed wetlands. In our study we also measured anammox and found that it was always <0.05{\%} of total nitrate reduction in these sediments.",
keywords = "Denitrification, DNRA, Ferrous Fe, Organic carbon, Wetland",
author = "Rahman, {Md Moklesur} and Roberts, {Keryn L.} and Grace, {Michael R.} and Kessler, {Adam J.} and Cook, {Perran L.M.}",
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Role of organic carbon, nitrate and ferrous iron on the partitioning between denitrification and DNRA in constructed stormwater urban wetlands. / Rahman, Md Moklesur; Roberts, Keryn L.; Grace, Michael R.; Kessler, Adam J.; Cook, Perran L.M.

In: Science of the Total Environment, Vol. 666, 20.05.2019, p. 608-617.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Role of organic carbon, nitrate and ferrous iron on the partitioning between denitrification and DNRA in constructed stormwater urban wetlands

AU - Rahman, Md Moklesur

AU - Roberts, Keryn L.

AU - Grace, Michael R.

AU - Kessler, Adam J.

AU - Cook, Perran L.M.

PY - 2019/5/20

Y1 - 2019/5/20

N2 - Denitrification (DNF) and dissimilatory nitrate reduction to ammonium (DNRA) are two competing nitrate reduction pathways that remove or recycle nitrogen, respectively. However, factors controlling the partitioning between these two pathways are manifold and our understanding of these factors is critical for the management of N loads in constructed wetlands. An important factor that controls DNRA in an aquatic ecosystem is the electron donor, commonly organic carbon (OC) or alternatively ferrous iron and sulfide. In this study, we investigated the role of natural organic carbon (NOC) and acetate at different OC/NO 3 − ratios and ferrous iron on the partitioning between DNF and DNRA using the 15 N-tracer method in slurries from four constructed stormwater urban wetlands in Melbourne, Australia. The carbon and nitrate experiments revealed that DNF dominated at all OC/NO 3 − ratios. The higher DNF and DNRA rates observed after the addition of NOC indicates that nitrate reduction was enhanced more by NOC than acetate. Moreover, addition of NOC in slurries stimulated DNRA more than DNF. Interestingly, slurries amended with Fe 2+ showed that Fe 2+ had significant control on the balance between DNF and DNRA. From two out of four wetlands, a significant increase in DNRA rates (p < .05) at the cost of DNF in the presence of available Fe 2+ suggests DNRA is coupled to Fe 2+ oxidation. Rates of DNRA increased 1.5–3.5 times in the Fe 2+ treatment compared to the control. Overall, our study provides direct evidence that DNRA is linked to Fe 2+ oxidation in some wetland sediments and highlights the role of Fe 2+ in controlling the partitioning between removal (DNF) and recycling (DNRA) of bioavailable N in stormwater urban constructed wetlands. In our study we also measured anammox and found that it was always <0.05% of total nitrate reduction in these sediments.

AB - Denitrification (DNF) and dissimilatory nitrate reduction to ammonium (DNRA) are two competing nitrate reduction pathways that remove or recycle nitrogen, respectively. However, factors controlling the partitioning between these two pathways are manifold and our understanding of these factors is critical for the management of N loads in constructed wetlands. An important factor that controls DNRA in an aquatic ecosystem is the electron donor, commonly organic carbon (OC) or alternatively ferrous iron and sulfide. In this study, we investigated the role of natural organic carbon (NOC) and acetate at different OC/NO 3 − ratios and ferrous iron on the partitioning between DNF and DNRA using the 15 N-tracer method in slurries from four constructed stormwater urban wetlands in Melbourne, Australia. The carbon and nitrate experiments revealed that DNF dominated at all OC/NO 3 − ratios. The higher DNF and DNRA rates observed after the addition of NOC indicates that nitrate reduction was enhanced more by NOC than acetate. Moreover, addition of NOC in slurries stimulated DNRA more than DNF. Interestingly, slurries amended with Fe 2+ showed that Fe 2+ had significant control on the balance between DNF and DNRA. From two out of four wetlands, a significant increase in DNRA rates (p < .05) at the cost of DNF in the presence of available Fe 2+ suggests DNRA is coupled to Fe 2+ oxidation. Rates of DNRA increased 1.5–3.5 times in the Fe 2+ treatment compared to the control. Overall, our study provides direct evidence that DNRA is linked to Fe 2+ oxidation in some wetland sediments and highlights the role of Fe 2+ in controlling the partitioning between removal (DNF) and recycling (DNRA) of bioavailable N in stormwater urban constructed wetlands. In our study we also measured anammox and found that it was always <0.05% of total nitrate reduction in these sediments.

KW - Denitrification

KW - DNRA

KW - Ferrous Fe

KW - Organic carbon

KW - Wetland

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U2 - 10.1016/j.scitotenv.2019.02.225

DO - 10.1016/j.scitotenv.2019.02.225

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