Role of ompR-dependent genes in Salmonella typhimurium virulence: Mutants deficient in both ompC and ompF are attenuated in vivo

S. N. Chatfield, C. J. Dorman, C. Hayward, G. Dougan

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Abstract

A Salmonella typhimurium strain harboring stable mutations in both ompC and ompF was constructed from the mouse-virulent strain S. typhimurium SL1344. When administered orally to BALB/c mice the strain was attenuated, with the 50% lethal dose (LD50) reduced by approximately 1,000-fold. However,the intravenous LD50 was reduced only by approximately 10-fold. The ompC ompF mutant persisted in murine tissues for several weeks following oral challenge, and mice immunized with this mutant were well protected against challenge with virulent SL1344. A strain harboring a stable mutation in tppB behaved in a manner similar to that of strain SL1344 in vivo,while a strain harboring mutations in ompC, ompF, and tppB behaved as an ompC ompF mutant in vivo, indicating that the tppB operon is not required for virulence in S. typhimurium.

Original languageEnglish
Pages (from-to)449-452
Number of pages4
JournalInfection and Immunity
Volume59
Issue number1
Publication statusPublished - 1 Jan 1991
Externally publishedYes

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