Role of monocytes in mediating HIV-specific antibody-dependent cellular cytotoxicity

Marit Kramski, A Schorcht, Angus Phillip Rayner Johnston, Gregor F Lichtfuss, S Jegaskanda, Robert De Rose, Ivan Stratov, Anthony D Kelleher, M A French, Robert John Center, Anthony Jaworowski, Stephen J Kent

Research output: Contribution to journalArticleResearchpeer-review

48 Citations (Scopus)

Abstract

We assessed the effector cells that mediate ADCC in this assay. Backgating analysis and phenotyping of CFSE-PKH26 + revealed that the RFADCC assay s readout mainly represents CD3-CD14 + monocytes taking up the PKH26 dye. This was confirmed for 53 HIV + plasma-purified IgG samples when co-cultured with PBMC from three separate healthy donors
Original languageEnglish
Pages (from-to)51 - 61
Number of pages11
JournalJournal of Immunological Methods
Volume384
Issue number1-2
DOIs
Publication statusPublished - 2012

Cite this